CAJ | 학술논문

使用扫描电镜对牙鲆、大菱鲆和条斑星鲽的精子和卵子表面形态结构进行比较研究.观察结果表明,3种精子形态结构在核前凹陷位置、精子头部形状、中段线粒体数及鞭毛长度4个方面存在明显差异;3种卵子表面形态结构中,牙鲆和大菱鲆比较相似,而条斑星鲽成熟卵子壳膜具有4点特异性:外表面粗糙、有点状和斑块状突起、网纹较深;壳膜上微小孔孔径差距大;卵膜厚度最厚为(3.87 ± 0.24) μm,约为牙鲆和大菱鲆卵膜厚度的2倍;受精孔的管道螺旋层为4~6层,约为前两者的1/2.条斑星鲽与牙鲆和大菱鲆的精卵表面形态结构差异明显.
사용소묘전경대아평、대릉평화조반성접적정자화란자표면형태결구진행비교연구.관찰결과표명,3충정자형태결구재핵전요함위치、정자두부형상、중단선립체수급편모장도4개방면존재명현차이;3충란자표면형태결구중,아평화대릉평비교상사,이조반성접성숙란자각막구유4점특이성:외표면조조、유점상화반괴상돌기、망문교심;각막상미소공공경차거대;란막후도최후위(3.87 ± 0.24) μm,약위아평화대릉평란막후도적2배;수정공적관도라선층위4~6층,약위전량자적1/2.조반성접여아평화대릉평적정란표면형태결구차이명현.
The surface morphological structures of sperm and mature egg of three species flatfish Japanese flounder Paralichthys olivaceus ,turbot Scophthalmus maximus L,and Verasper moseri were studied by scanning electron microscopy. Comparing results demonstrated that there are four obvious differences in sperm surface structures among three species flatfish,including the location of nucleus anterior pit,the shape of sperm head,the number of mitochondria in midpiece and length of the flagellum. But in the surface morphology of mature egg of three species flatfish,it is similar essentially among Japanese flounder and turbot,excepting stripe stars flounder. Four structure characteristics of the shell membrane are observed in V. moseri ,they are a rough outer surface,various microspores in size,diameter as well as the most thick shell membrane(3.87±0.24) μm which is twice thicker than Japanses flounder and turbot and spiral pipe layer in micropyle only for 4-6 layers about half of Japanses Flounder and turbot. It was concluded that the difference was obvious in the surface morphology of sperm and mature egg between three species of Flatfish,and stripe stars flounder as genetic distance of the farthest Pleuronectidae fish,have significant level.