CAJ | 학술논문

以结球甘蓝胞质雄性不育系99-A及其保持系99-B为试材,采用石蜡切片技术,对结球甘蓝胞质雄性不育系的花粉败育过程进行了研究.结果表明:与雄性不育保持系99-B相比,不育系99-A的减数分裂行为基本正常,大多能发育至四分体时期,少数能发育到小孢子阶段;但在减数分裂前期,不育系99-A绒毡层细胞便开始肥大生长和液泡化,到小孢子时期绒毡层细胞肥大和液泡化达到最大程度,药室内的小孢子相互粘连、集聚,随后小孢子便退化解体,药室干缩,彻底败育.
이결구감람포질웅성불육계99-A급기보지계99-B위시재,채용석사절편기술,대결구감람포질웅성불육계적화분패육과정진행료연구.결과표명:여웅성불육보지계99-B상비,불육계99-A적감수분렬행위기본정상,대다능발육지사분체시기,소수능발육도소포자계단;단재감수분렬전기,불육계99-A융전층세포편개시비대생장화액포화,도소포자시기융전층세포비대화액포화체도최대정도,약실내적소포자상호점련、집취,수후소포자편퇴화해체,약실간축,철저패육.
Using paraffin slice techniques, the process of meiotic behavior and microspore abortion in cytoplasmic male-sterile line 99-A of cabbage were studied. The result showed that most of microspore mother cells could develop into the tetrad stage and a few could develop to the single nucleus microspore stage in male-sterile line 99-A. But compared with the male container line 99-B, the tapetum cells in the male sterile line began to overgrow with vacuole during early meiotic prophase and the overgrowth reached the peak before or after microspore formation, and at the same time the microspores were adhered and centralized one another, finally the anther became shrinking and the microspores disaggregating completely.