中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2008年
9期
608-611
,共4页
姜种弋%韩天权%蒋兆彦%王建承%吴卫泽%雷若庆%蔡劬%姜志宏%蔡杏兴%张圣道
薑種弋%韓天權%蔣兆彥%王建承%吳衛澤%雷若慶%蔡劬%薑誌宏%蔡杏興%張聖道
강충익%한천권%장조언%왕건승%오위택%뢰약경%채구%강지굉%채행흥%장골도
胆结石%小肠%胆固醇吸收
膽結石%小腸%膽固醇吸收
담결석%소장%담고순흡수
Cholelithiasis%Intestine%Cholesterol absorption No30672042,30700310)
目的 该研究通过分析胆石病人小肠胆固醇吸收相关基因的表达情况探讨小肠胆固醇吸收的遗传凶素在胆石病发生中的作用.方法 研究包括10例胆囊结石病人和7例无胆石症的对照.实时定量PCR法测定近段空肠黏膜胆固醇吸收相关基因mRNA的表达量.结果 胆石组小肠胆固醇吸收相关基因的mRNA表达量与对照组没有统计学差异(NPCIL1:1.15±0.60 vs 0.80±0.29;ABCG5:3.11±2.70 vs 1.92±1.38;ABCG8:3.65±2.08 vs 2.85±1.33;ACAT2:0.30±0.25vs 0.20±0.16;MTTP:12.35±8.10 vs 8.22±5.74,P>0.05).结论 胆石病人小肠胆固醇吸收相关基因表达差异不是胆石病发生的主要遗传因素.
目的 該研究通過分析膽石病人小腸膽固醇吸收相關基因的錶達情況探討小腸膽固醇吸收的遺傳兇素在膽石病髮生中的作用.方法 研究包括10例膽囊結石病人和7例無膽石癥的對照.實時定量PCR法測定近段空腸黏膜膽固醇吸收相關基因mRNA的錶達量.結果 膽石組小腸膽固醇吸收相關基因的mRNA錶達量與對照組沒有統計學差異(NPCIL1:1.15±0.60 vs 0.80±0.29;ABCG5:3.11±2.70 vs 1.92±1.38;ABCG8:3.65±2.08 vs 2.85±1.33;ACAT2:0.30±0.25vs 0.20±0.16;MTTP:12.35±8.10 vs 8.22±5.74,P>0.05).結論 膽石病人小腸膽固醇吸收相關基因錶達差異不是膽石病髮生的主要遺傳因素.
목적 해연구통과분석담석병인소장담고순흡수상관기인적표체정황탐토소장담고순흡수적유전흉소재담석병발생중적작용.방법 연구포괄10례담낭결석병인화7례무담석증적대조.실시정량PCR법측정근단공장점막담고순흡수상관기인mRNA적표체량.결과 담석조소장담고순흡수상관기인적mRNA표체량여대조조몰유통계학차이(NPCIL1:1.15±0.60 vs 0.80±0.29;ABCG5:3.11±2.70 vs 1.92±1.38;ABCG8:3.65±2.08 vs 2.85±1.33;ACAT2:0.30±0.25vs 0.20±0.16;MTTP:12.35±8.10 vs 8.22±5.74,P>0.05).결론 담석병인소장담고순흡수상관기인표체차이불시담석병발생적주요유전인소.
Objective To determine whether genetic variations in intestinal cholesterol absorp-tion are related to the pathogenesis of gallstone formation in humans. Methods Mucosa of the proxi-mal jejunum and serum samples were collected in ten cholesterol gallstone patients (GS) and seven gallstone-free controls (GSF). The mRNA expression levels of genes were analyzed by real-time PCR.Biliary lipids composition was measured in those subjects for whom gallbladder bile was available (GS,n=5;GSF, n=3). Results Control subjects were matched to gallstone patients for age (GS vs.GSF: 52.5±18.9 vs. 67.0±9.9, P>0.05) and body mass index (GS vs. GSF: 21.19±2.04 vs.19.14±1.35, P<0.05). Cholesterol saturation index was increased by 47%in GS (0.99±0.31)compared with GSF (0.67±0.22). But this was not statistically significant due to few samples.There was no difference in mRNA expression levels of NPC1L1, ABCG5, ABCG8, ACAT2, and MTTP between GS and GSF (GS vs. GSF: NPC1LI: 1.155±0.60 vs. 0. 80±0.29;ABCG5: 3.11±2.70 vs. 1.92±1.38;ABCG8: 3.65±2.08 vs. 2.85±1.33;ACAT2: 0.30±0.25vs. 0.20±0.16;MTTP: 12.35±8.10 vs. 8.22±5.74, P>0.05). Conclusion There was no difference in mRNA expression levels of the known genes involved in intestinal cholesterol absorption between gallstonc pa-tients and gallstone-free controls. The genetic variations in intestinal cholesterol absorption might not be related to the pathogenesis of gallstone disease in Chinese patients.
