国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2011年
3期
140-144
,共5页
时黎明%赵立平%何璐%鄢文慧%乔海暄
時黎明%趙立平%何璐%鄢文慧%喬海暄
시려명%조립평%하로%언문혜%교해훤
蛋白质组学%Sut黑色素细胞%xCT
蛋白質組學%Sut黑色素細胞%xCT
단백질조학%Sut흑색소세포%xCT
Proteomics%Sut melanocytes%xCT
目的 为研究xCT缺陷引起细胞生长抑制的机理提供理论依据.方法 提取xCT缺陷的Sut黑色素细胞和野生型Mela黑色素细胞2组细胞总蛋白,分别进行二维凝胶电泳,分离2组细胞总蛋白.以Mela细胞做对照,以PDQuest软件分析Sut细胞蛋白质表达谱的变化.选择部分表达量明显变化的蛋白质进行质谱分析,将质谱分析结果输入MASCOT软件,在SWISS-PROT数据库中搜索,鉴定出差异蛋白质.结果 和野生型Mela细胞相比,Sut细胞中有20个蛋白质发生了明显变化,其中10个蛋白质表达上调,10个蛋白质表达下调,分别选取上调蛋白质点和下调蛋白质点各4个进行鉴定.鉴定结果显示表达量上调的蛋白质为:Tubulin alpha-1b,S100-A6,Nucleoside,S-formylglutathione;表达量下调的蛋白质为:Calumenin,NDRG1,DPYSL2,14 ku未知蛋白.结论 获得了一些与xCT缺陷相关的蛋白质,为研究xCT缺陷引起的Sut细胞生长抑制机理提供理论依据.
目的 為研究xCT缺陷引起細胞生長抑製的機理提供理論依據.方法 提取xCT缺陷的Sut黑色素細胞和野生型Mela黑色素細胞2組細胞總蛋白,分彆進行二維凝膠電泳,分離2組細胞總蛋白.以Mela細胞做對照,以PDQuest軟件分析Sut細胞蛋白質錶達譜的變化.選擇部分錶達量明顯變化的蛋白質進行質譜分析,將質譜分析結果輸入MASCOT軟件,在SWISS-PROT數據庫中搜索,鑒定齣差異蛋白質.結果 和野生型Mela細胞相比,Sut細胞中有20箇蛋白質髮生瞭明顯變化,其中10箇蛋白質錶達上調,10箇蛋白質錶達下調,分彆選取上調蛋白質點和下調蛋白質點各4箇進行鑒定.鑒定結果顯示錶達量上調的蛋白質為:Tubulin alpha-1b,S100-A6,Nucleoside,S-formylglutathione;錶達量下調的蛋白質為:Calumenin,NDRG1,DPYSL2,14 ku未知蛋白.結論 穫得瞭一些與xCT缺陷相關的蛋白質,為研究xCT缺陷引起的Sut細胞生長抑製機理提供理論依據.
목적 위연구xCT결함인기세포생장억제적궤리제공이론의거.방법 제취xCT결함적Sut흑색소세포화야생형Mela흑색소세포2조세포총단백,분별진행이유응효전영,분리2조세포총단백.이Mela세포주대조,이PDQuest연건분석Sut세포단백질표체보적변화.선택부분표체량명현변화적단백질진행질보분석,장질보분석결과수입MASCOT연건,재SWISS-PROT수거고중수색,감정출차이단백질.결과 화야생형Mela세포상비,Sut세포중유20개단백질발생료명현변화,기중10개단백질표체상조,10개단백질표체하조,분별선취상조단백질점화하조단백질점각4개진행감정.감정결과현시표체량상조적단백질위:Tubulin alpha-1b,S100-A6,Nucleoside,S-formylglutathione;표체량하조적단백질위:Calumenin,NDRG1,DPYSL2,14 ku미지단백.결론 획득료일사여xCT결함상관적단백질,위연구xCT결함인기적Sut세포생장억제궤리제공이론의거.
Objective To investigate the mechanism of Sut melanocytes growth inhibition in response to xCT -deficiency. Methods TTotal proteins were extracted from xCT-deficient Sut melanocytes and wild melanocytes, respectively, and were separated by 2-dimensional electrophoresis. Altered expression profile of proteins of Sut melanocytes was analyzed by PDQuest software and compared with that of wild melanocytes.Proteins with significant change were chosen to be identified by mass spectrometry and database query.Results Twenty proteins in Sut melanocytes altered significantly compared with wild melanocytes. Ten of the proteins were up-regulated, while the other tens were down-regulated. Four proteins from both up-regulated and down-regulated were identified respectively: up-regulated proteins were Tubulin alpha-1b, S100-A6,Nucleoside, S-formylglutathione, and down-regulated proteins were Calumenin,NDRG1 ,DPYSL2, 14kDa unknown protein. Conclusion The identification of the xCT-deficiency related proteins may provide supporting evidence for the mechanism research of Sut melanocytes' growth inhibition caused by xCT-deficiency.
