CAJ | 학술논문

目的探讨急性支气管哮喘(简称哮喘)小鼠CD4+T细胞表面白介素23受体(IL-23R)的表达及在哮喘发病中的作用.方法建立急性哮喘小鼠模型,免疫磁珠分离小鼠脾源性CD4+T细胞,培养24 h后,检测CD4+T细胞表面IL-23R的表达、Th17细胞的阳性率及细胞培养上清液中的IL-17水平.结果哮喘小鼠脾脏CD4+T细胞表面IL-23R的表达明显高于正常组(P＜0.01);哮喘小鼠脾脏CD4+T细胞中Th17细胞的阳性率明显高于正常组(P＜0.01);哮喘小鼠脾脏CD4+T细胞分泌IL-17浓度明显高于正常组(P＜0.01);小鼠脾脏CD4+T细胞表面IL-23R的表达与Th17细胞的阳性率和IL-17的浓度呈正相关.结论 IL-23R在急性哮喘的发病机制中可能起重要作用.
목적 탐토급성지기관효천(간칭효천)소서CD4+T세포표면백개소23수체(IL-23R)적표체급재효천발병중적작용.방법 건립급성효천소서모형,면역자주분리소서비원성CD4+T세포,배양24 h후,검측CD4+T세포표면IL-23R적표체、Th17세포적양성솔급세포배양상청액중적IL-17수평.결과 효천소서비장CD4+T세포표면IL-23R적표체명현고우정상조(P＜0.01);효천소서비장CD4+T세포중Th17세포적양성솔명현고우정상조(P＜0.01);효천소서비장CD4+T세포분비IL-17농도명현고우정상조(P＜0.01);소서비장CD4+T세포표면IL-23R적표체여Th17세포적양성솔화IL-17적농도정정상관.결론 IL-23R재급성효천적발병궤제중가능기중요작용.
Objective To detect the expression of IL-23 receptor on spleen CD4+ T lymphocytes in mouse model of acute bronchial asthma (asthma)as well as its significance in the pathogenesis. Methods Spleen CD4+ T lymphocytes origin from spleen of mouse model of acute asthma isolated by immunomagnetic beads and they were used to determine the expression of IL-23 receptor mRNA by realtime PCR,the level of IL-23 receptor protein by Western blotting,the ratio of positive Th17 cells by flow cytometer and the production of IL-17 by ELISA. Results The expression of IL-23 receptor,the ratio of Thl7 cells and the production of IL-17 on Spleen CD4+ T lymphocytes in asthmatic model was obviously higher than that of the control group. The expression of IL-23 receptor on spleen CD4+ T lymphocytes positively correlated with the ratio of positive Th17 cells and the production of IL-17. Conclusions IL-23receptor possibly plays an important role in the pathogenesis of asthma.