CAJ | 학술논문

目的观察白蚁菌圃醇提液对α-突触核蛋白(α-synuclein)转基因帕金森病(PD)果蝇模型攀爬能力及寿命的影响以探讨其扰PD作用机制. 方法采用乙醇提取白蚁菌圃,通过果蝇添食不同浓度白蚁菌圃醇提液(每100 g培养液添加1、10和100 mg),观察比较PD果蝇的攀爬能力、寿命；用刚果红染色观察脑组织病理改变. 结果白蚁菌圃醇提取液提高PD果蝇攀爬能力,在其羽化后18～36 d最显著,攀爬能力随白蚁菌圃醇提取液浓度升高及果蝇年龄的增长而增高.白蚁菌圃醇提取液延长PD果蝇的寿命雄果蝇寿命较PD果蝇对照延长31.82％～3.34％(均P＜0.05),并呈剂量依赖关系.白蚁菌圃醇提液作用后PD果蝇脑组织中的神经细胞数明显增加,空泡减少,且与PD果蝇的行为及寿命变化一致. 结论白蚁菌囿醇提取液改善PD果蝇行为及延长PD果蝇寿命.
목적 관찰백의균포순제액대α-돌촉핵단백(α-synuclein)전기인파금삼병(PD)과승모형반파능력급수명적영향이탐토기우PD작용궤제. 방법 채용을순제취백의균포,통과과승첨식불동농도백의균포순제액(매100 g배양액첨가1、10화100 mg),관찰비교PD과승적반파능력、수명；용강과홍염색관찰뇌조직병리개변. 결과 백의균포순제취액제고PD과승반파능력,재기우화후18～36 d최현저,반파능력수백의균포순제취액농도승고급과승년령적증장이증고.백의균포순제취액연장PD과승적수명웅과승수명교PD과승대조연장31.82％～3.34％(균P＜0.05),병정제량의뢰관계.백의균포순제액작용후PD과승뇌조직중적신경세포수명현증가,공포감소,차여PD과승적행위급수명변화일치. 결론 백의균유순제취액개선PD과승행위급연장PD과승수명.
Objective To observe the eftccts of the extract of termitarium on the climbing ability and life span of Parkinson disease (PD)drosophila(Ddc GAL4/+ ;UAS-wild type α-synuclein/I ),and to explore its mechanism of anti- PD. Methods The termitarium was extracted by ethanol.PD drosophilas overexpressed α-synuclein were fed with 1,10 and 100 mg of termitarium per 100 g culture medium.The climbing ability and life span of drosophilas and pathological changes of its brain tissue (Congo red staining) were observed and compared among different concentrations. Results Termitarium enhanced the climbing ability of PD drosophilas and the most significant improvement appeared from 18 d to 36 d after eclosion,increasing with more termitarium concentrations and growing age of PD drosophilas.Termitarium also prolonged the life span among which male and female drosophila were longer 31.82 ％～ 3.34 ％,1.47 ％～ 3.34 ％ as compared with PD drosophila control (all P＜0.05).The brain cells number in the PD drosophilas were increased and empty bubble was decreased by rermitarium, accompanying with improvement of behavior and life span.Conclusions Termitarium can improve behavior and prolong life span of PD drosophilas.