CAJ | 학술논문

目的检测不同恶性程度胶质瘤组织NAD(P)H氧化酶(NOX)的表达及其产物ROS的含量,探讨NOX及细胞内ROS与胶质瘤增殖及恶性程度的关系.方法收集南方医科大学珠江医院神经外科白2007年8月至2010年8月手术切除的人脑胶质瘤标本30例,其中低级别胶质瘤(Ⅰ和Ⅱ级)10例、Ⅲ级胶质瘤10例和Ⅳ级胶质瘤10例,另取10例行内减压术的脑损伤患者的正常脑组织作为对照组.RT-PCR检测4组脑组织NOX1～5 mRNA的表达,流式细胞仪测定脑组织ROS的含量,Western blotting和免疫荧光染色分别检测脑组织NOX蛋白的表达和分布.结果 4组脑标本NOX1～5 mRNA、ROS含量均不同,差异均有统计学意义(P＜0.05),且NOX4mRNA 、ROS含量在正常对照组、低级别胶质瘤(Ⅰ～Ⅱ级)、Ⅲ级胶质瘤和Ⅳ级胶质瘤中的表达逐渐增加,差异均有统计学意义(P＜0.05);Westenm blotting和免疫荧光染色结果显示NOX在对照组标本中呈低表达,在胶质瘤中呈高表达,且胶质瘤恶性程度越高,表达水平越高.结论 NOX的表达及ROS含量与胶质瘤的恶性程度有关,可能对胶质瘤的发生和恶性增殖起重要作用.
목적 검측불동악성정도효질류조직NAD(P)H양화매(NOX)적표체급기산물ROS적함량,탐토NOX급세포내ROS여효질류증식급악성정도적관계.방법 수집남방의과대학주강의원신경외과백2007년8월지2010년8월수술절제적인뇌효질류표본30례,기중저급별효질류(Ⅰ화Ⅱ급)10례、Ⅲ급효질류10례화Ⅳ급효질류10례,령취10례행내감압술적뇌손상환자적정상뇌조직작위대조조.RT-PCR검측4조뇌조직NOX1～5 mRNA적표체,류식세포의측정뇌조직ROS적함량,Western blotting화면역형광염색분별검측뇌조직NOX단백적표체화분포.결과 4조뇌표본NOX1～5 mRNA、ROS함량균불동,차이균유통계학의의(P＜0.05),차NOX4mRNA 、ROS함량재정상대조조、저급별효질류(Ⅰ～Ⅱ급)、Ⅲ급효질류화Ⅳ급효질류중적표체축점증가,차이균유통계학의의(P＜0.05);Westenm blotting화면역형광염색결과현시NOX재대조조표본중정저표체,재효질류중정고표체,차효질류악성정도월고,표체수평월고.결론 NOX적표체급ROS함량여효질류적악성정도유관,가능대효질류적발생화악성증식기중요작용.
[Objective] To detect the expression of NAD(P)H oxidase (Nox) and the content of ROS in different malignant gliomas,and explore the influence of Nox and intracellular ROS levels in the survival,proliferation,and malignant phenotype of gliomas.[Methods]Thirty human glioma specimens (10 with grade Ⅰ and I1,10 with grade II and 10 with grade IV),performed resection in our hospital from August 2007 to August 2010,were collected in our study;another 10 normal brain tissues were collected as controls.Real time PCR was used to detect the mRNA expressions of Nox(1-5);ROS level was detected by flow cytometry and Nox4 protein level was detected by immunofluorescence and Western blotting.[Results] The Noxl-5 mRNA and protein levels and ROS content were significantly different between each 2 groups (P＜0.05);Nox4 mRNA expression and ROS level significantly increased following the increment of malignancy degrees (controls＜low-grade gliomas＜grade III gliomas＜ grade IV gliomas,P＜0.05);the Nox protein expression was low in controls while that in gliomas was high,and the higher malignancy of the tumors,the higher expression levels of the tumors.[Conclusion] Nox expression and ROS content have significantly positive relations with the malignancy of the tumors,which indicates that Nox expression and ROS content might paly important roles in the occurrence of glioma and malignant proliferation.