CAJ | 학술논문

目的观察消退素E1( RvE1)对急性肺损伤(ALI)小鼠炎症反应的影响.方法建立小鼠ALI模型,12h后处死,观察RyE1治疗对肺组织形态、湿/干比、肺泡灌洗液(BALF)肿瘤坏死因子-α( TNF-α)含量、组织匀浆核因子-κB( NF-κB)蛋白水平的影响.结果给予RvE1治疗后,肺组织损伤明显减轻；RYE1治疗组肺湿/干比值(4.637 ±0.125) g/g、TNF-α含量(217.2 ±30.1)ng/L较ALI模型对照组肺湿/干比值(4.906±0.176) g/g和TNF-α含量(372.2±20.6)ng/L均明显降低,差异有统计学意义(P ＜0.05)；RvE1治疗组肺组织匀浆中NF-κB蛋白表达水平较ALI模型对照组明显下降.结论 RvE1能减轻肺部炎症反应,从而减轻肺组织损伤,对ALI具有保护作用.
목적 관찰소퇴소E1( RvE1)대급성폐손상(ALI)소서염증반응적영향.방법 건립소서ALI모형,12h후처사,관찰RyE1치료대폐조직형태、습/간비、폐포관세액(BALF)종류배사인자-α( TNF-α)함량、조직균장핵인자-κB( NF-κB)단백수평적영향.결과 급여RvE1치료후,폐조직손상명현감경；RYE1치료조폐습/간비치(4.637 ±0.125) g/g、TNF-α함량(217.2 ±30.1)ng/L교ALI모형대조조폐습/간비치(4.906±0.176) g/g화TNF-α함량(372.2±20.6)ng/L균명현강저,차이유통계학의의(P ＜0.05)；RvE1치료조폐조직균장중NF-κB단백표체수평교ALI모형대조조명현하강.결론 RvE1능감경폐부염증반응,종이감경폐조직손상,대ALI구유보호작용.
Objective To evaluate the intluence ot resolvin E1 ( RvE1 ) on intlammatory response in mice with acute lung injury (ALI).Methods The animal model of ALI was established. After 12 h,histological changes,wet-to-dry (W/D) weight ratio of the lungs and the contents of tumor necrosis factorα (TNF-α) in bronchoalveolar lavage fluid (BALF) and nuclear factor-κB (NF-κB) in lung homogenate were observed.Results Histological study showed the lung injury was significantly alleviated in RvE1-treated group.W/D ratio (4.637 ±0.125) g/g and contents of TNF-α (217.2 ±30.1 ) ng/L in BALF of RvE1-treated group were significantly decreased as compared with ALI model group [for W/D ratio:(4.906 ± 0.176) g/g,and for contents of TNF-α:(372.2 ± 20.6) ng/L] ( P ＜ 0.05 ).The level of NF-κB in RvE1-treated group was significantly lower than in ALI model control group.Conclusion RvE1 can inhibit inflammatory response and alleviate lung injury in mice with ALI.