中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2009年
8期
967-969
,共3页
李甲初%杨欣荣%崔越宏%王志军%刘康达%周康
李甲初%楊訢榮%崔越宏%王誌軍%劉康達%週康
리갑초%양흔영%최월굉%왕지군%류강체%주강
癌,肝细胞%KLF8%侵袭
癌,肝細胞%KLF8%侵襲
암,간세포%KLF8%침습
Carcinoma,hepatocellular%KLF8%Invasiveness
目的 观察不同肝癌细胞株及肝癌组织中Krappel样因子8(KLF8)的表达,并探讨其意义.方法 用逆转录.聚合酶链反应(RT-PCR)、Western blot、免疫化学方法分别检测不同肝癌细胞株及肝(癌)组织中KLF8的mRNA和蛋白质水平.结果 KLF8的mRNA及蛋白质水平随肝癌细胞株侵袭转移潜能的增强而明显升高(P<0.05);正常肝组织(6例)、无转移肝癌组织(17例)、有转移肝癌组织(6例)中KLF8 mRNA的相对表达量分别为0.2377±0.0658、0.4683±0.2073、0.6669±0.0239(P<0.05);在正常肝组织和肝硬化组织中KLF8蛋白表达的阳性率分别为16.7%(1/6)和33.3%(2/6),均为弱阳性,在肝癌组织中强阳性率为81.8%(27/33),肝癌转移灶中强阳性率为83.3%(5/6)(P<0.01).结论 KLF8可能参与肝癌的发生和侵袭转移.
目的 觀察不同肝癌細胞株及肝癌組織中Krappel樣因子8(KLF8)的錶達,併探討其意義.方法 用逆轉錄.聚閤酶鏈反應(RT-PCR)、Western blot、免疫化學方法分彆檢測不同肝癌細胞株及肝(癌)組織中KLF8的mRNA和蛋白質水平.結果 KLF8的mRNA及蛋白質水平隨肝癌細胞株侵襲轉移潛能的增彊而明顯升高(P<0.05);正常肝組織(6例)、無轉移肝癌組織(17例)、有轉移肝癌組織(6例)中KLF8 mRNA的相對錶達量分彆為0.2377±0.0658、0.4683±0.2073、0.6669±0.0239(P<0.05);在正常肝組織和肝硬化組織中KLF8蛋白錶達的暘性率分彆為16.7%(1/6)和33.3%(2/6),均為弱暘性,在肝癌組織中彊暘性率為81.8%(27/33),肝癌轉移竈中彊暘性率為83.3%(5/6)(P<0.01).結論 KLF8可能參與肝癌的髮生和侵襲轉移.
목적 관찰불동간암세포주급간암조직중Krappel양인자8(KLF8)적표체,병탐토기의의.방법 용역전록.취합매련반응(RT-PCR)、Western blot、면역화학방법분별검측불동간암세포주급간(암)조직중KLF8적mRNA화단백질수평.결과 KLF8적mRNA급단백질수평수간암세포주침습전이잠능적증강이명현승고(P<0.05);정상간조직(6례)、무전이간암조직(17례)、유전이간암조직(6례)중KLF8 mRNA적상대표체량분별위0.2377±0.0658、0.4683±0.2073、0.6669±0.0239(P<0.05);재정상간조직화간경화조직중KLF8단백표체적양성솔분별위16.7%(1/6)화33.3%(2/6),균위약양성,재간암조직중강양성솔위81.8%(27/33),간암전이조중강양성솔위83.3%(5/6)(P<0.01).결론 KLF8가능삼여간암적발생화침습전이.
Objective To investigate the expression and role of Kruppel-like factor 8 (KLF8) in hepatocellular carcinoma (HCC).Methods The KLF8 mRNA and protein levels in different HCC cell lines and HCC tissues were determined by RT-PCR, immunochemistry and Western blot respectively.Resuits Metastatic potential HCC cell lines had higher KLF8 mRNA and protein levels than primary HCC cell lines (P <0.05).Relative KLF8 mRNA levels in normal liver tissues (6 cases) ,HCC tissues (17 cases), and recurrent HCC tissues (6 cases) were 0.2377 ± 0.0658, 0.4683 ± 0.2073, and 0.6669 ± 0.0239, respectively (P < 0.05).Metastasis specimens exhibited the highest level of KLF8 expression (5/6,83.3 % strong positive), and primary tumor tissue specimens (27/33,81.8%) had higher level of KLF8 expression than normal (1/6,16.7%) and liver cirrhosis tissue specimens (2/6,33.3%), (P < 0.01).Conclusion Abnormally activated KLF8 expression may contribute to HCC development and progression.
