CAJ | 학술논문

目的：探讨聚酯型儿茶素对人肝癌SMMC-7721细胞的生长抑制作用及其机制。方法：应用MTT法、集落形成试验、同位素掺入试验、细胞内cAMP/cGMP浓度测定、原位杂交等方法进行检测。结果：50mg*L-1聚酯型儿茶素对SMMC-7721细胞的生长、集落形成有明显的抑制作用；3H-TdR、3H-Leu掺入试验证明其可明显抑制细胞DNA和蛋白质合成；聚酯型儿茶素作用后，SMMC-7721细胞内cAMP及cGMP浓度明显增加，细胞的c-myc基因mRNA水平表达明显降低，而p53基因mRNA水平表达明显升高。结论：聚酯型儿茶素对肝癌SMMC-7721细胞的生长有明显的抑制作用，此作用与其抑制细胞DNA和蛋白质合成、升高细胞内cAMP浓度和抑制c-myc基因表达、增强p53基因表达有关。
목적：탐토취지형인다소대인간암SMMC-7721세포적생장억제작용급기궤제。방법：응용MTT법、집락형성시험、동위소참입시험、세포내cAMP/cGMP농도측정、원위잡교등방법진행검측。결과：50mg*L-1취지형인다소대SMMC-7721세포적생장、집락형성유명현적억제작용；3H-TdR、3H-Leu참입시험증명기가명현억제세포DNA화단백질합성；취지형인다소작용후，SMMC-7721세포내cAMP급cGMP농도명현증가，세포적c-myc기인mRNA수평표체명현강저，이p53기인mRNA수평표체명현승고。결론：취지형인다소대간암SMMC-7721세포적생장유명현적억제작용，차작용여기억제세포DNA화단백질합성、승고세포내cAMP농도화억제c-myc기인표체、증강p53기인표체유관。
Objective　To investigate the inhibitory effect of theasinesin on the growth of SMMC-7721 human hepatic carcinoma cells line and its mechanism. Methods　MTT assay, colony formation test,3H-TdR,3H-Leu incorporation test, determination of the intracellular cAMP and cGMP level, in situ hybridization were used. Results　Theasinesin at 50mg*L-1 could significantly inhibit the growth and colony formation of SMMC-7721 cells. Theasinesin could strongly inhibit DNA and protein synthesis and increase intracellular cAMP levels. C-myc gene expression was significantly decreased and p53 gene increased strongly in a dose-dependent manner. Conclusions　Theasinesin had significant inhibitory effects on the growth of SMMC-7721 cells. Its mechanisms may involve the inhibition of DNA and protein synthesis, the elevation of intracellular cAMP levels, inhibition of c-myc gene expression and enhancement of p53 gene expression.