天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2009年
7期
574-577
,共4页
张玉环%邢艳玲%刘艳华%廉信
張玉環%邢豔玲%劉豔華%廉信
장옥배%형염령%류염화%렴신
雷公藤%皮炎,特应性%白细胞介素类%干扰素Ⅱ型%RNA,信使%逆转录聚合酶链反应
雷公籐%皮炎,特應性%白細胞介素類%榦擾素Ⅱ型%RNA,信使%逆轉錄聚閤酶鏈反應
뢰공등%피염,특응성%백세포개소류%간우소Ⅱ형%RNA,신사%역전록취합매련반응
ripterygium wilfordii%dermatitis,atopic%interleukins%interferon type Ⅱ%RNA,messenger%reverse transcriptase polymerase chain reaction
目的:探讨雷公藤多苷对特应性皮炎(AD)患者IL-2、IL-4、IFN-γ和IL-10细胞因子表达的影响.方法:RT-PCR方法测定与不同浓度雷公藤多苷共同培养AD患者外周血单个核细胞(PBMC)IL-2、IL-4、IFN-γ和IL-10mRNA表达.结果:(1)IL-2 mRNA表达,高浓度组与对照组、低浓度组、中浓度组比较差别均有统计学意义(P＜0.05或P＜0.01).中浓度组与对照组比较差别有统计学意义(P＜0.01).(2)IL-4 mRNA表达,对照组与高浓度组、中浓度组和低浓度组比较差别均有统计学意义(P＜0.01).(3)IFN-γmRNA表达,高浓度组与对照组、低浓度组和中浓度组,中浓度组与对照组之间差别有统计学意义(P＜0.05或P＜0.01).(4)IL-10mRNA表达,高浓度组与中浓度组之间差别无统计学意义(P＞0.05),其余各组差别均有统计学意义(P＜0.01或P＜0.05).结论:雷公藤多苷对体外培养AD患者PBMC中IL-2、IL-4、IFN-γ、IL-10 mRNA均有抑制作用,但在低浓度时对高表达的IL-4和IL-10有抑制作用,对低表达IL-2、IFN-γ抑制作用不显著.
目的:探討雷公籐多苷對特應性皮炎(AD)患者IL-2、IL-4、IFN-γ和IL-10細胞因子錶達的影響.方法:RT-PCR方法測定與不同濃度雷公籐多苷共同培養AD患者外週血單箇覈細胞(PBMC)IL-2、IL-4、IFN-γ和IL-10mRNA錶達.結果:(1)IL-2 mRNA錶達,高濃度組與對照組、低濃度組、中濃度組比較差彆均有統計學意義(P＜0.05或P＜0.01).中濃度組與對照組比較差彆有統計學意義(P＜0.01).(2)IL-4 mRNA錶達,對照組與高濃度組、中濃度組和低濃度組比較差彆均有統計學意義(P＜0.01).(3)IFN-γmRNA錶達,高濃度組與對照組、低濃度組和中濃度組,中濃度組與對照組之間差彆有統計學意義(P＜0.05或P＜0.01).(4)IL-10mRNA錶達,高濃度組與中濃度組之間差彆無統計學意義(P＞0.05),其餘各組差彆均有統計學意義(P＜0.01或P＜0.05).結論:雷公籐多苷對體外培養AD患者PBMC中IL-2、IL-4、IFN-γ、IL-10 mRNA均有抑製作用,但在低濃度時對高錶達的IL-4和IL-10有抑製作用,對低錶達IL-2、IFN-γ抑製作用不顯著.
목적:탐토뢰공등다감대특응성피염(AD)환자IL-2、IL-4、IFN-γ화IL-10세포인자표체적영향.방법:RT-PCR방법측정여불동농도뢰공등다감공동배양AD환자외주혈단개핵세포(PBMC)IL-2、IL-4、IFN-γ화IL-10mRNA표체.결과:(1)IL-2 mRNA표체,고농도조여대조조、저농도조、중농도조비교차별균유통계학의의(P＜0.05혹P＜0.01).중농도조여대조조비교차별유통계학의의(P＜0.01).(2)IL-4 mRNA표체,대조조여고농도조、중농도조화저농도조비교차별균유통계학의의(P＜0.01).(3)IFN-γmRNA표체,고농도조여대조조、저농도조화중농도조,중농도조여대조조지간차별유통계학의의(P＜0.05혹P＜0.01).(4)IL-10mRNA표체,고농도조여중농도조지간차별무통계학의의(P＞0.05),기여각조차별균유통계학의의(P＜0.01혹P＜0.05).결론:뢰공등다감대체외배양AD환자PBMC중IL-2、IL-4、IFN-γ、IL-10 mRNA균유억제작용,단재저농도시대고표체적IL-4화IL-10유억제작용,대저표체IL-2、IFN-γ억제작용불현저.
Objective: To study the effect of tripterygium wilfordii polyglycosidium (TWP) on atopie dermatitis (AD) by detecting the expression of IL-2,IL-4,IFN-γ and IL-IO mRNA. Methods: RT-RCR was used to detect the mRNA of IL-2,IL-4, IFN-γ and IL-10 in peripheral blood mononuelear cells (PBMC)of AD cultured with different concentrations of TWP.ResuRs:(1)The expression of IL-2 was significantly different between the high concentration group and other groups(P＜0.05 or P＜0.01 ), and between the control group and the middle concentration group (P＜0.01). (2)The expression of IL-4 was significantly different between the three different concentration groups (P＜0.01 ). (3) The expression of IFN-γmRNA was significantly different between the high concentration group and the control, the low, the middle concentration groups (P＜0.05 or P＜0.01 ). (4)There was no significant difference in expression of IL-10 mRNA between the high concentration group and the middle concentration group (P＞0.05), there was significant difference among the other groups (P＜0.05 or P＜0.01).Conclusion: TWP can suppress the expression of IL-2, IL-4, IFN-γand IL-10 mRNA, but the low concentration of TWP can suppress the high-level expression of IL-4 and IL-10mRNA in vitro.
