CAJ | 학술논문

利用海水琼脂、TCBS和MRS培养基对斜带石斑鱼Epinephelus coioides 幼鱼消化道和养殖水体中的细菌进行分离纯化,采用生理生化鉴定结合16S rRNA基因测序进行细菌鉴定,探讨了幼鱼消化道中的菌群与养殖水体中菌群的关系.结果表明:幼鱼消化道中可培养细菌总数(9.0×10~6 cfu/g)高于养殖水体中可培养细菌总数(5.4×10~5 cfu/mL),幼鱼消化道中细菌种类也明显多于养殖水体;在幼鱼消化道和养殖水体中均检测到副溶血弧菌、哈维氏弧菌、短小芽孢杆菌、克劳氏芽孢杆菌、鲍氏不动杆菌、嗜冷杆菌和洋葱伯克霍尔德菌等7种细菌.细菌系统进化分析结果表明,这些细菌隶属于β-变形杆菌纲、γ-变形杆菌纲和芽孢杆菌纲.幼鱼消化道和养殖水体的优势菌中均有芽孢杆菌和嗜冷杆菌,提示芽孢杆菌和嗜冷杆菌能通过养殖水体很好地进入消化道.3种乳酸菌(乳酸乳球菌、干酪乳杆菌和屎肠球菌)仅见于幼鱼消化道,且仅占消化道菌群数量的0.70%.弧菌在消化道和养殖水体中所占比例均很小,分别仅占细菌总数的1.07%和0.10%.
이용해수경지、TCBS화MRS배양기대사대석반어Epinephelus coioides 유어소화도화양식수체중적세균진행분리순화,채용생리생화감정결합16S rRNA기인측서진행세균감정,탐토료유어소화도중적균군여양식수체중균군적관계.결과표명:유어소화도중가배양세균총수(9.0×10~6 cfu/g)고우양식수체중가배양세균총수(5.4×10~5 cfu/mL),유어소화도중세균충류야명현다우양식수체;재유어소화도화양식수체중균검측도부용혈호균、합유씨호균、단소아포간균、극로씨아포간균、포씨불동간균、기랭간균화양총백극곽이덕균등7충세균.세균계통진화분석결과표명,저사세균대속우β-변형간균강、γ-변형간균강화아포간균강.유어소화도화양식수체적우세균중균유아포간균화기랭간균,제시아포간균화기랭간균능통과양식수체흔호지진입소화도.3충유산균(유산유구균、간락유간균화시장구균)부견우유어소화도,차부점소화도균군수량적0.70%.호균재소화도화양식수체중소점비례균흔소,분별부점세균총수적1.07%화0.10%.
The microbiota in the gut of juvenile grouper Epinephelus coioides and aquaculture water were isolated and purified by marine agar, TCBS and MRS media. The traits of the cultivable bacteria were analysed by physiological and biochemical tests in combination with 16S rRNA gene sequence analysis. The results showed that there were higher total cultivable bacterial number(9.0×10~6 cfu/g)and species in gut of the grouper than those in the aquaculture water(5.4×10~5 cfu/mL).Seven bacterial species, however, were found in both the gut and aquaculture water, i.e. Vibrio parahaemolyticus, Vibrio harveyi, Bacillus pumilus, Bacillus clausii, Acinetobacter baumannii,Psychroacter sp. and Burkholderia cepacia, which were classified into 3 groups γ-proteobacteria, β-proteobacteria and Bacilli. Bacillus and Psychrobacter invading into the gut via the aquaculture water were found to be the predominant bacteria in the gut and aquaculture water. Three species of lactic acid bacteria, Lactococcus lactis, Lactobacillus casei and Enterococcus faecium were isolated only from the gut, but comprising only 0.70% of the total bacteria. Furthermore, the amount of Vibrio in the gut and aquaculture water was very low, accounting for only 1.07% and 0.10% of the total bacteria, respectively.