CAJ | 학술논문

目的比较纤维蛋白凝胶与几丁质对骨髓间充质干细胞(BMSCs)向软骨细胞分化的影响,探讨三维支架与软骨组织工程种子细胞BMSCs分化的关系. 方法 BMSCs与几丁质、纤维蛋白凝胶形成复合物,分别体外培养及植入大鼠关节软骨缺损部位.体外培养14d后,进行HE染色、甲苯胺蓝及Ⅱ型胶原免疫组织化学染色;体内培养2周、4周、6周后,对移植物进行形态学观察,表达软骨特异蛋白分析及BMSCs体内示踪.统计学分析BMSCs向软骨分化情况. 结果体外培养部分,BMSCs-纤维蛋白凝胶组和BMSCs-几丁质组的Ⅱ型胶原免疫组织化学染色阳性率与对照组无显著差异;体内移植部分,BMSCs-纤维蛋白凝胶组的甲苯胺蓝染色与Ⅱ型胶原免疫组织化学染色积分吸光度(IA)变化率与对照组有显著差异,其他组别软骨分化与对照组无显著差异. 结论在体外纤维蛋白凝胶或几丁质诱导BMSCs向软骨细胞分化的作用很弱,在体内BMSCs-纤维蛋白凝胶可促进BMSCs分化成类软骨细胞.
목적 비교섬유단백응효여궤정질대골수간충질간세포(BMSCs)향연골세포분화적영향,탐토삼유지가여연골조직공정충자세포BMSCs분화적관계. 방법 BMSCs여궤정질、섬유단백응효형성복합물,분별체외배양급식입대서관절연골결손부위.체외배양14d후,진행HE염색、갑분알람급Ⅱ형효원면역조직화학염색;체내배양2주、4주、6주후,대이식물진행형태학관찰,표체연골특이단백분석급BMSCs체내시종.통계학분석BMSCs향연골분화정황. 결과 체외배양부분,BMSCs-섬유단백응효조화BMSCs-궤정질조적Ⅱ형효원면역조직화학염색양성솔여대조조무현저차이;체내이식부분,BMSCs-섬유단백응효조적갑분알람염색여Ⅱ형효원면역조직화학염색적분흡광도(IA)변화솔여대조조유현저차이,기타조별연골분화여대조조무현저차이. 결론 재체외섬유단백응효혹궤정질유도BMSCs향연골세포분화적작용흔약,재체내BMSCs-섬유단백응효가촉진BMSCs분화성류연골세포.
Objective We compare the effects of fibrinogen gel and chitin on BMSCs to chondrocytes differentiation in order to explore the relationship between three-dimensional scaffold and cartilage tissue engineering seed cells BMSCs differentiation. Methods BMSCs together with chitin and fibrin gel complexes were cultured in vitro and implanted into rat's articular cartilage defect location. After 14 days in vitro culture, HE staining, toluidine blue staining and type II collagen immunohistochemical staining were performed;After transplantation in vivo, for 2 weeks, 4 weeks and 6 weeks, morphology observations, expression of cartilage-specific protein analysis and BMSCs in vivo tracer method were performed. We analyzed the differentiation of BMSCs into chondrocytes by statistical methods. Results The comparison of positive cell rate of type II collagen immunohistochemical staining in BMSCs-fibrin gel group and BMSCs-chitin group which were cultured in vitro, showed no significant difference with control group. Integrated absorbance(IA) change rate of toluidine blue staining and type II collagen immunohistochemical staining in BMSCs-fibrin gel group which was cultured in vivo, showed significantly different with other groups and control group.Conclusion The results showed that in vitro fibrin gel or chitin has very weak induction of BMSCs to cartilage differentiation, while in vivo BMSCs-fibrin gel can facilitrate BMSCs to differentiate into chondrocyte-like cells.