CAJ | 학술논문

目的通过模拟腹腔镜二氧化碳(CO_2)气腹环境,研究CO_2气腹对肾透明细胞癌细胞增殖和凋亡的影响,探讨腹腔镜手术治疗肾脏肿瘤的安全性.方法选用人肾透明细胞癌细胞株RCC-949在99%CO_2、1%O_2、21℃和15 mmHg(1 mmHg=0.133 kPa)条件下模拟腹腔镜CO_2气腹环境离体培养1、2、4 h,应用脱氧核苷酸末端转移酶介导的生物素化dUTP缺口末端标记技术(TUNEL)检测RCC-949细胞在CO_2气腹环境干预后的凋亡;采用噻唑蓝法(MTT法)检测RCC-949细胞在CO_2气腹环境干预后的增殖.结果正常对照组、低氧1 h组、低氧2 h组和低氧4 h组增殖的光密度值分别为0.348±0.051、0.372±0.082、0.389±0.110和0.378±0.114,凋亡细胞百分率分别为(3.2±0.5)%、(3.4±0.3)%、(3.8±0.4)%和(4.3±0.5)%,各组的差异均无统计学意义(P值均0.05).结论在模拟腹腔镜CO_2气腹环境中培养4 h之内,RCC-949细胞的增殖和凋亡水平无显著改变.由此推测,腹腔镜CO_2气腹环境并不增加肿瘤细胞种植转移的机会,腹腔镜手术治疗肾脏肿瘤安全、有效.
목적 통과모의복강경이양화탄(CO_2)기복배경,연구CO_2기복대신투명세포암세포증식화조망적영향,탐토복강경수술치료신장종류적안전성.방법 선용인신투명세포암세포주RCC-949재99%CO_2、1%O_2、21℃화15 mmHg(1 mmHg=0.133 kPa)조건하모의복강경CO_2기복배경리체배양1、2、4 h,응용탈양핵감산말단전이매개도적생물소화dUTP결구말단표기기술(TUNEL)검측RCC-949세포재CO_2기복배경간예후적조망;채용새서람법(MTT법)검측RCC-949세포재CO_2기복배경간예후적증식.결과 정상대조조、저양1 h조、저양2 h조화저양4 h조증식적광밀도치분별위0.348±0.051、0.372±0.082、0.389±0.110화0.378±0.114,조망세포백분솔분별위(3.2±0.5)%、(3.4±0.3)%、(3.8±0.4)%화(4.3±0.5)%,각조적차이균무통계학의의(P치균0.05).결론 재모의복강경CO_2기복배경중배양4 h지내,RCC-949세포적증식화조망수평무현저개변.유차추측,복강경CO_2기복배경병불증가종류세포충식전이적궤회,복강경수술치료신장종류안전、유효.
bjective To investigate the influence of laparoscopic CO_2 pneumoperitoneum on the proliferation and apoptosis of renal cell carcinoma (RCC-949) cells in vitro, so as to assess the safety of laparoscopic surgery for renal tumors. Methods Human clear cell renal cell carcinoma (RCC-949) cells were cultured for 1 hour, 2 hours and 4 hours in a simulated laparoscopic CO_2 pneumoperitoneum environment at 21 ℃, 99% CO_2, 1% O_2 and 15 mmHg (1 mmHg = 0. 133 kPa). The proliferation and apoptosis of RCC-949 cells cultured in the CO_2 pneumoperitoneum environment were examined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and methyl thiazolyl tetrazolium (MTT) assay, respectively. Results The optical density (OD) values of cell proliferation in the control group, hypoxia 1 h group, hypoxia 2 h group and hypoxia 4 h group were 0. 348 ± 0. 051, 0. 372 ± 0. 082, 0. 389 ± 0. 110 and 0. 378 ± 0. 114, respectively. Meanwhile, the apoptosis rates in the control group, hypoxia 1 h group, hypoxia 2 h group and hypoxia 4 h group were (3.2±0.5)%, (3.4±0.3)%, (3.8±0.4)% and (4.3±0.5)%, respectively. There were no significant differences in OD values or the apoptosis rates among all the groups (all P 0. 05). Conclusion The proliferation and apoptosis rates of RCC-949 cells are not significantly changed within 4 hours of culture in the simulated laparoscopic CO_2 pneumoperitoneum. Thus, we speculate that laparoscopic CO_2 pneumoperitoneum does not promote tumor metastasis and implantation of RCC-949 cells, and laparoscopic surgery is safe and effective for renal tumors.