CAJ | 학술논문

发现新小分子DNA嵌插剂是改进肿瘤化疗的有效途径之一.抗肿瘤活性评价模型和嵌插机制研究模型是发现新小分子DNA嵌插剂的重要基础.本文描述了细胞和动物以外的模型,包括DOCK评分、紫外(UV)、荧光、圆二色散(CD)、相对粘度、熔点曲线、小分子与CT DNA堆叠动力学常数、小分子与CT DNA嵌插动力学常数、小分子嵌插剂和CT DNA复合物的稳定常数和凝胶色谱电泳模型.
발현신소분자DNA감삽제시개진종류화료적유효도경지일.항종류활성평개모형화감삽궤제연구모형시발현신소분자DNA감삽제적중요기출.본문묘술료세포화동물이외적모형,포괄DOCK평분、자외(UV)、형광、원이색산(CD)、상대점도、용점곡선、소분자여CT DNA퇴첩동역학상수、소분자여CT DNA감삽동역학상수、소분자감삽제화CT DNA복합물적은정상수화응효색보전영모형.
For improving the cancer chemotherapy the discovery of new DNA intercalator is one of the effective approaches. The investigations have demonstrated the utility of the models in both evaluation of anti-tumor activity and identification of intercalation mechanism. This paper describes the DOCK score, ultraviolet(UV), fluorescence, circular dichroism(CD), relative viscosity, melting curve, kinetics in calf thymus(CT) DNA stacking of small molecule intercalator, kinetics in CT DNA intercalation of small molecule intercalator, interaction stabilizing the stacking complex of small molecule intercalator and CT DNA and the gel electrophoresis based models as well as their use in the identification of small molecule intercalating towards DNA.