CAJ | 학술논문

利用RAPD标记技术,对辽宁8个杂交榛子主栽品种进行指纹分析。从60个10碱基随机引物中筛选重复性好的3个引物OPF05、OPG01、OPH07,选取7个条带作为品种鉴别的RAPD标记,以此构建榛子优良品种的指纹图谱。根据扩增图谱可以将8个品种区分,研究结果为榛子品种的区别与鉴定提供了一种有效方法。
이용RAPD표기기술,대료녕8개잡교진자주재품충진행지문분석。종60개10감기수궤인물중사선중복성호적3개인물OPF05、OPG01、OPH07,선취7개조대작위품충감별적RAPD표기,이차구건진자우량품충적지문도보。근거확증도보가이장8개품충구분,연구결과위진자품충적구별여감정제공료일충유효방법。
RAPD-based fingerprinting was carried out on 8 cultivars of Corylus spp.which had been approved by the Liaoning Inspection Committee of Superior Tree Species.Each of the cultivars was identified by fingerprints.Among the 60 random oligonueleotide primers used for RAPD analysis,OPF05,OPG01 and OPH07 could produce stable DNA patterns and were selected for further study.Seven RAPD markers were obtained and DNA fingerprints were made for eight hazel cultivars.According to the DNA fingerprints,the eight cultivars can be distinguished successfully.DNA fingerprints would provide effective tools for the identification of the cultivars.