CAJ | 학술논문

目的探讨西藏地区藏族人基质金属蛋白酶-9(MMP-9)基因多态性与慢性阻塞性肺疾病(COPD)易感性的关系.方法采用病例对照研究方法,收集180例吸烟COPD患者和180名健康对照组(96名吸烟组和84名非吸烟组).应用聚合酶链反应-限制性片段长度多态性技术检测MMP-9(1562C/T)基因型频率在病例组与对照组的差别.结果 MMP-9(1562)的基因型CC和CT+TT的频率在180例COPD患者中分别是66.67%、33.33%;96例健康吸烟对照组中的频率是87.5%、12.5%;健康不吸烟对照组的频率是92.9%、7.1%.C等位基因频率在COPD组、健康吸烟组和健康不吸烟组分别是83.33%、92.7%和96.43%;T等位基因的频率分别是16.67%、7.3%和3.57%.结论 MMP-9(1562C/T)基因型可增加西藏地区藏族人COPD易感性,且这种突变可能与吸烟相关密切.
목적 탐토서장지구장족인기질금속단백매-9(MMP-9)기인다태성여만성조새성폐질병(COPD)역감성적관계.방법 채용병례대조연구방법,수집180례흡연COPD환자화180명건강대조조(96명흡연조화84명비흡연조).응용취합매련반응-한제성편단장도다태성기술검측MMP-9(1562C/T)기인형빈솔재병례조여대조조적차별.결과 MMP-9(1562)적기인형CC화CT+TT적빈솔재180례COPD환자중분별시66.67%、33.33%;96례건강흡연대조조중적빈솔시87.5%、12.5%;건강불흡연대조조적빈솔시92.9%、7.1%.C등위기인빈솔재COPD조、건강흡연조화건강불흡연조분별시83.33%、92.7%화96.43%;T등위기인적빈솔분별시16.67%、7.3%화3.57%.결론 MMP-9(1562C/T)기인형가증가서장지구장족인COPD역감성,차저충돌변가능여흡연상관밀절.
Objective To explore whether the matrix metalloproteinases-9 (MMP-9) polymorphism was associated with the susceptibility to chronic obstructive pulmonary diseas (COPD) in Ethnic Tibetan population. Methods Case-Control study was employed and 180 COPD (current smokers) and 180 control subjects (96 smokers, and 84 non-smokers) were selected. Genotyping was determined by using PCRRFLP both in case and controls subjects. Results The frequencies of genotype CC and CT+TT in COPD were 66.67% and 33.33% respectively. In smoker control group were 87.5% and 12.5% respectively and in non-smoker control group were 92.9% and 7.1% respectively. The frequencies of C allele in COPD,smoker control group and non-smoker control group were 83.33 %, 92.7 % and 96.43 % respectively. The frequencies of T allele in three groups above were 16.67% ,7. 3% and 3.57% respectively. Conclusions The genetic polymorphism of MMP-9 (1562 C/T) was associated with the susceptibility to COPD in Ethnic Tibetan population and appearance of this mutation is closely associated with smoking.