CAJ | 학술논문

分子遗传学方法在儿童白血病分型、分层诊断中起重要作用.大部分白血病存在一系列与特定形态、临床特点相关的染色体重排.融合基因作为基因结构重排的产物,是白血病特异性的分子标志,目前已广泛应用于白血病的分型诊断、微小残留病的诊断、评估预后及靶向治疗.迄今为止,儿童白血病在分子遗传学领域的各类检测方法从传统的PCR,发展至多重逆转录PCR、实时定量逆转录PCR、荧光原位杂交技术、比较基因组杂交、流式细胞学技术、全基因组测序等.
분자유전학방법재인동백혈병분형、분층진단중기중요작용.대부분백혈병존재일계렬여특정형태、림상특점상관적염색체중배.융합기인작위기인결구중배적산물,시백혈병특이성적분자표지,목전이엄범응용우백혈병적분형진단、미소잔류병적진단、평고예후급파향치료.흘금위지,인동백혈병재분자유전학영역적각류검측방법종전통적PCR,발전지다중역전록PCR、실시정량역전록PCR、형광원위잡교기술、비교기인조잡교、류식세포학기술、전기인조측서등.
Molecular genetics plays an irreplaceable role in the diagnosis of MICM classification of childhood leukemia.The majority of leukemia cases have one of a series of chromosome abnormalities,many of which are associated with specific morphological and clinical characteristics.Fusion gene as a structural rearrangement of the gene product are considered to be leukemia-specific molecular markers.Therefore,the detection of fusion gene can be used for diagnosis of leukemia,diagnosis of minirnal residual disease,prognosis and targeted therapy.Originally,the detection of various types of childhood leukemia in the field of molecular genetics detected by traditional methods,such as polymerase chain reaction.In the past few decades,there were various methods developed,such as multiplex reverse transcription polymerase chain reaction,real-time quantitative reverse transcriptase polymerase chain reaction,fluorescence in situ hybridization,comparative genomic hybridization,flow cytometry technology,whole-genome sequencing.