慢性阻塞性肺疾病血清基质金属蛋白酶-9和组织型金属蛋白酶抑制物-1的检测及其意义
만성조새성폐질병혈청기질금속단백매-9화조직형금속단백매억제물-1적검측급기의의
Serum levels and significance of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in patients with chronic obstructive pulmonary disease
  • 万方数据
    国际呼吸杂志 국제호흡잡지
    INTERNATIONAL JOURNAL OF RESPIRATION
    2011年 20期 1533-1537 ,共5页

    王嘉%孙武装%于卫芳%王丽红%李珠

    왕가%손무장%우위방%왕려홍%리주

    慢性阻塞性肺疾病%蛋白酶/抗蛋白酶失衡%基质金属蛋白酶-9%组织型金属蛋白酶抑制物-1%吸烟%肺功能 慢性阻塞性肺疾病%蛋白酶/抗蛋白酶失衡%基質金屬蛋白酶-9%組織型金屬蛋白酶抑製物-1%吸煙%肺功能
    만성조새성폐질병%단백매/항단백매실형%기질금속단백매-9%조직형금속단백매억제물-1%흡연%폐공능
    Chronic obstructive pulmonary disease%Imbalance of protease and antiprotease%Matrix metalloproteinase-9%Tissue inhibitor of metalloproteinase-1%Smoking%Pulmonary function
    目的 检测慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)急性加重期、稳定期患者和健康对照者血清中基质金属蛋白酶-9 (matrix metalloproteinase-9,MMP-9)和组织型金属蛋白酶抑制物-1 (tissue inhibitor of metalloproteinase-1,TIMP-1)的水平变化,统计分析其与肺功能指标和吸烟等危险因素的关系,探讨蛋白酶/抗蛋白酶失衡在COPD发病中的意义.方法 收集2007年12月至2010年10月河北医科大学第一医院呼吸内科住院的COPD患者56例和健康对照21名的血清标本.COPD病例又分为急性加重期30例和稳定期26例两组.采用酶联免疫吸附法检测各组血清中MMP-9、TIMP-1水平.结果 1.①COPD急性加重期组MMP-9水平显著高于稳定期组、正常对照组,差异有统计学意义(t =3.24,4.22,P＜0.01);稳定期组高于正常对照组,差异有统计学意义(t=2.08,P＜0.05).②COPD急性加重期组TIMP-1水平高于稳定期组、正常对照组,差异有统计学意义(t =2.02,3.65,P＜0.05,0.01);稳定期组高于正常对照组,差异有统计学意义(t=2.11,P＜0.05).③COPD急性加重期组MMP-9/TIMP-1比值高于稳定期组、正常对照组,差异有统计学意义(t=2.51,2.11,P＜0.05);稳定期组低于正常对照组,差异无统计学意义(t=-0.39,P＞0.05).2.COPD急性加重期组MMP-9水平与FEV1％ pred呈负相关(r=-0.37,P＜0.05);与RV/TLC或吸烟程度呈正相关(r =0.39,0.41,P＜0.05).COPD稳定期血清MMP-9水平与FEV1％pred或RV/TLC无相关性(r =0.25,-0.26,P＞0.05);与吸烟程度正相关(r=0.50,P＜0.05).3.COPD急性加重期组TIMP-1水平与FEV1％ pred呈明显负相关(r=-0.51,P＜0.01);与RV/TLC或吸烟程度无相关性(r =0.36,0.28,P＞0.05).COPD稳定期组TIMP-1水平与FEV1％ pred、RV/TLC或吸烟程度无相关性(r =0.18,-0.15,0.17,P＞0.05).4.COPD急性加重期及稳定期组血清MMP-9/TIMP-1与FEV1％ pred、RV/TLC或吸烟程度均无相关性(r=0.19,-0.02,0.06,0.17,-0.23,0.38,P＞0.05).结论 1.MMP-9、TIMP-1及MMP-9/TIMP-1在COPD发病中起重要作用.2.MMP-9、TIMP-1可直接影响COPD急性加重期患者的肺功能水平.3.MMP-9水平与吸烟量关系密切,可能在吸烟导致的COPD发病中起重要作用.
    목적 검측만성조새성폐질병(chronic obstructive pulmonary disease,COPD)급성가중기、은정기환자화건강대조자혈청중기질금속단백매-9 (matrix metalloproteinase-9,MMP-9)화조직형금속단백매억제물-1 (tissue inhibitor of metalloproteinase-1,TIMP-1)적수평변화,통계분석기여폐공능지표화흡연등위험인소적관계,탐토단백매/항단백매실형재COPD발병중적의의.방법 수집2007년12월지2010년10월하북의과대학제일의원호흡내과주원적COPD환자56례화건강대조21명적혈청표본.COPD병례우분위급성가중기30례화은정기26례량조.채용매련면역흡부법검측각조혈청중MMP-9、TIMP-1수평.결과 1.①COPD급성가중기조MMP-9수평현저고우은정기조、정상대조조,차이유통계학의의(t =3.24,4.22,P＜0.01);은정기조고우정상대조조,차이유통계학의의(t=2.08,P＜0.05).②COPD급성가중기조TIMP-1수평고우은정기조、정상대조조,차이유통계학의의(t =2.02,3.65,P＜0.05,0.01);은정기조고우정상대조조,차이유통계학의의(t=2.11,P＜0.05).③COPD급성가중기조MMP-9/TIMP-1비치고우은정기조、정상대조조,차이유통계학의의(t=2.51,2.11,P＜0.05);은정기조저우정상대조조,차이무통계학의의(t=-0.39,P＞0.05).2.COPD급성가중기조MMP-9수평여FEV1％ pred정부상관(r=-0.37,P＜0.05);여RV/TLC혹흡연정도정정상관(r =0.39,0.41,P＜0.05).COPD은정기혈청MMP-9수평여FEV1％pred혹RV/TLC무상관성(r =0.25,-0.26,P＞0.05);여흡연정도정상관(r=0.50,P＜0.05).3.COPD급성가중기조TIMP-1수평여FEV1％ pred정명현부상관(r=-0.51,P＜0.01);여RV/TLC혹흡연정도무상관성(r =0.36,0.28,P＞0.05).COPD은정기조TIMP-1수평여FEV1％ pred、RV/TLC혹흡연정도무상관성(r =0.18,-0.15,0.17,P＞0.05).4.COPD급성가중기급은정기조혈청MMP-9/TIMP-1여FEV1％ pred、RV/TLC혹흡연정도균무상관성(r=0.19,-0.02,0.06,0.17,-0.23,0.38,P＞0.05).결론 1.MMP-9、TIMP-1급MMP-9/TIMP-1재COPD발병중기중요작용.2.MMP-9、TIMP-1가직접영향COPD급성가중기환자적폐공능수평.3.MMP-9수평여흡연량관계밀절,가능재흡연도치적COPD발병중기중요작용.
    Objective To detect the levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in serum of chronic obstructive pulmonary disease (COPD)group and health control group,and analyzed the relationship between the levels and pulmonary function indicator or quantity of smoking.Methods Fifty six cases of serum samples with COPD,and twenty one cases of serum samples from health control were collected and analyzed.The COPD group was divided into two groups,one of acute exacerbation phase (AECOPD),and the other of stable phase (STCOPD).The levels of serum MMP-9 and TIMP-1 in aboved groups were measured by enzyme-linked immunosorbent assay (ELISA) method.Results 1.① The level of MMP-9 in AECOPD group is significantly higher than which in STCOPD group or health control group ( t =3.24,4.22,P ＜0.01).The level of MMP-9 in STCOPD group is significantly higher than which in health control group ( t =2.08,P ＜ 0.05).②The level of TIMP-1 in AECOPD group are significantly higher than which in STCOPD group or health control group ( t =2.02,3.65,P ＜ 0.05,0.01).There are significant difference between the level in STCOPD with the level in health group( t =2.11,P ＜0.05).③The ratio of MMP-9/TIMP-1 in AECOPD group are higher than which in STCOPD group or health control group ( t =2.51,2.11,P ＜0.05).There are no significant difference between the level in STCOPD with the level in health group ( t =-0.39,P ＞0.05).2.There is a negative correlatin between the levels of MMP-9 with FEV1 ％pred in AECOPD group (r =-0.37,P ＜0.05).There are positive correlation between the levels of MMP-9 with RV/TLC or the amount of smoking in AECOPD ( r =0.39,0.41,P ＜0.05).In STCOPD group,there are no correlation between the level of MMP-9 with FEV1 ％ pred or RV/TLC ( r =0.25,-0.26,P ＞0.05),and a positive correlation between the level and the amount of smoking ( r =0.50,P ＜0.05).3.In AECOPD group,there are a negative correlation between the level of TIMP-1 with FEV1 ％pred ( r =-0.51,P ＜0.01),and no correlation between the level with RV/ TLC or smoking ( r =0.36,0.28,P ＞0.05).In STCOPD group,there are no correlation between the level of TIMP-1 with FEV1 ％pred,RV/TLC or the level with smoking ( r =0.18,-0.15,0.17,P ＞0.05).4.There are no correlation between the ratio of MMP-9/TIMP-1 with FEV1 ％pred,RV/TLC or the level with smoking in AECOPD group and STCOPD group ( r =0.19,-0.02,0.06,0.17,-0.23,0.38,P ＞0.05).Conclusions 1.MMP-9,TIMP-1 and MMP-9/TIMP-1 play the important roles in the pathogenesis of COPD.2.MMP-9 and TIMP-1 can influence pulmonary function index of AECOPD patients directly.3.There is a significance correlation between the quantity of smoking and the levels of MMP-9,and which may be the important roles in the pathogenesis of COPD caused by smoking.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문