CAJ | 학술논문

背景姜黄素能清除机体中产生的自由基和超氧负离子,从而抑制脂质过氧化反应.研究证实姜黄素具有抗晶状体氧化损伤的作用,但其对年龄相关性白内障的作用尚不清楚.目的观察姜黄素对实验性大鼠硒性白内障的影响.方法选用12日龄健康SD大鼠30只,按随机数字表法将其随机分为空白对照组、模型对照组、姜黄素组,每组10只.除空白对照组外,模型对照组和姜黄素组大鼠均采用皮下注射亚硒酸钠的方法建立硒性白内障模型,并于造模的同时,给予姜黄素组大鼠质量分数0.005％姜黄素灌胃,每日1次,共2周.于实验开始后第4、7、10、14天在裂隙灯显微镜下观察各组大鼠晶状体的混浊程度并进行评分.在实验结束后摘出大鼠晶状体,用生化测定法测定晶状体中丙二醛(MDA)及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶( GSH-Px)的活性.结果空白对照组大鼠晶状体在整个实验过程中保持透明.与模型对照组大鼠比较,姜黄素组大鼠Ⅲ、Ⅳ、V级晶状体混浊的时间明显延迟,差异均有统计学意义(P＜0.05).实验结束时,3个组MDA含量及SOD、GSH-Px活性的总体比较差异均有统计学意义(MDA:F=215.42,P＜0.01；SOD:F=46.83,P＜0.01；GSH-Px:F=44.29,P＜0.01).模型对照组、姜黄素组晶状体中SOD的活性明显低于空白对照组,差异均有统计学意义(P＜0.05),但姜黄素组SOD活性明显高于模型对照组(P＜0.05)；模型对照组和姜黄素组晶状体中MDA含量均高于空白对照组(P＜0.01),姜黄素组中MDA含量较模型对照组低(P＜0.01)；模型对照组GSH-Px活性较空白对照组显著下降(P＜0.01),姜黄素组GSH-Px活性较模型对照组高(P＜0.01).结论姜黄素能显著延缓大鼠硒性白内障的形成过程,但不能抑制硒性白内障的发生发展.姜黄素延缓大鼠硒性白内障形成的机制可能为提高大鼠晶状体的抗氧化能力.
배경 강황소능청제궤체중산생적자유기화초양부리자,종이억제지질과양화반응.연구증실강황소구유항정상체양화손상적작용,단기대년령상관성백내장적작용상불청초.목적 관찰강황소대실험성대서서성백내장적영향.방법선용12일령건강SD대서30지,안수궤수자표법장기수궤분위공백대조조、모형대조조、강황소조,매조10지.제공백대조조외,모형대조조화강황소조대서균채용피하주사아서산납적방법건립서성백내장모형,병우조모적동시,급여강황소조대서질량분수0.005％강황소관위,매일1차,공2주.우실험개시후제4、7、10、14천재렬극등현미경하관찰각조대서정상체적혼탁정도병진행평분.재실험결속후적출대서정상체,용생화측정법측정정상체중병이철(MDA)급초양화물기화매(SOD)、곡광감태과양화물매( GSH-Px)적활성.결과공백대조조대서정상체재정개실험과정중보지투명.여모형대조조대서비교,강황소조대서Ⅲ、Ⅳ、V급정상체혼탁적시간명현연지,차이균유통계학의의(P＜0.05).실험결속시,3개조MDA함량급SOD、GSH-Px활성적총체비교차이균유통계학의의(MDA:F=215.42,P＜0.01；SOD:F=46.83,P＜0.01；GSH-Px:F=44.29,P＜0.01).모형대조조、강황소조정상체중SOD적활성명현저우공백대조조,차이균유통계학의의(P＜0.05),단강황소조SOD활성명현고우모형대조조(P＜0.05)；모형대조조화강황소조정상체중MDA함량균고우공백대조조(P＜0.01),강황소조중MDA함량교모형대조조저(P＜0.01)；모형대조조GSH-Px활성교공백대조조현저하강(P＜0.01),강황소조GSH-Px활성교모형대조조고(P＜0.01).결론강황소능현저연완대서서성백내장적형성과정,단불능억제서성백내장적발생발전.강황소연완대서서성백내장형성적궤제가능위제고대서정상체적항양화능력.
Background Curcumin can eliminate free radical arising in the system and superoxide anion and further inhibit lipid peroxidation.Researches proved that curcumin has resisting effect on oxidative damage of lens,but its effect on the formation of age-related cataract is under clear.Objective This study was to investigate the effect of curcumin on the cataract induced by sodium selenite.Methods Thirty healthy clean SD rats of 12-day-old were selected.All the rats were randomly divided into the normal control group,model group and curcumin group and ten rats for every group.The selenite cataract models were established in the rats of model group and curcumin group by the injection of sodium selenite subcutaneously.Meanwhile,0.005 ％ curcumin lavaged the rats in the curcumin group daily for two weeks.Lens of the rats were examined under the slit lamp at 4,7,10,14 days after injection in all groups,and the opacification of lens was graded based on the He' criteria.The lenses of rats were obtained at the end of experiment and then the level of superoxide dismutase (SOD),the activities of malondialdehyde (MDA) and glutathione peroxidase(GSH-Px) in rat lens were detected using biochemical assay method.The use of experimental animals followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results The lenses were transparent throughout the experimental duration in the blank control group.Compared with model group,the development of the Ⅲ,Ⅳ,Ⅴ grade of cataract was slow down in the curcumin group with the significant difference( P＜0.05 ).The content of MDA,SOD and GSH-Px activity in lens appeared considerable differences among 3 groups ( MDA:F =215.42,P＜0.01 ; SOD:F =46.83,P＜ 0.01 ; GSH-Px:F=44.29,P＜0.01 ).The level of SOD was significantly reduced in the model group and the curcumin group compered with blank control group ( P ＜ 0.05 ) and that in the curcumin group was higher than the model group ( P＜0.05 ).The MDA level in the model group and the curcumin group was obviously rised in comparison with blank control group( P＜0.05 ),but that in the curcumin group lowed in comparison to model group( P＜0.05 ).GSH-Px activity in the curcumin group was significantly lower than that in blank control group and higher than that in the model group( P ＜ 0.05 ).Conclusions The curcumin can effectively delay rather than the cataract formation induced by sodium selenite in SD rat by improved the resistance of oxidation in the lens.