中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
5期
288-292
,共5页
龚智翔%曾玫%朱启镕%倪武%缪晓辉
龔智翔%曾玫%硃啟镕%倪武%繆曉輝
공지상%증매%주계용%예무%무효휘
轮状病毒感染%腹泻%基因型%儿童
輪狀病毒感染%腹瀉%基因型%兒童
륜상병독감염%복사%기인형%인동
Rotavirus infections%Diarrhea%Genotype%Child
目的 监测上海地区腹泻儿童A组轮状病毒的分子流行病学,为本地区轮状病毒疫苗的推广使用提供背景依据.方法 采集上海地区2008年8月至2009年7月门诊急性腹泻儿童的粪便标本910份,采用胶体金法检测A组轮状病毒抗原,使用套式反转录(RT)-PCR方法对轮状病毒进行G基因型和P基因型的分型检测.结果 910份粪便标本中,共检出A组轮状病毒阳性268份,占29.4％.全年均可检出A组轮状病毒,流行高峰为2008年10月至2009年1月,检出率为38.3％～70.5％.＜3岁婴幼儿的A组轮状病毒腹泻有244例,占91.0％,各年龄组检出率以12～23个月龄组最高,达36.6％.268株A组轮状病毒中,G基因型以G1最多,为65株(24.3％),以下依次为G3 40株(14.9％)、G混合型37株(13.8％)、G2 27株(10.1％)、G9 14株(5.2％)、G45株(1.9％),其他G分型5株(1.9％),G未分型75株(28.0％) ;P基因型以P[8]为主,为147株(54.9％),其次为P[4] 32株(11.9％)、P混合型6株(2.2％),其他P分型4株(1.5％),P未分型79株(29.5％).主要G/P基因型组合包括G1P[8] (13.4％)、G3P[8](13.4％)、G混合型P[8](10.1％)、G1P[4](8.2％)、G9P[8](2.2％)、G2P[4](1.9％)、G1P混合型(1.9％).结论 A组轮状病毒是上海地区婴幼儿腹泻的常见病原体,流行高峰为秋冬季节.目前注册的轮状病毒疫苗覆盖了上海地区绝大部分轮状病毒G/P基因型.
目的 鑑測上海地區腹瀉兒童A組輪狀病毒的分子流行病學,為本地區輪狀病毒疫苗的推廣使用提供揹景依據.方法 採集上海地區2008年8月至2009年7月門診急性腹瀉兒童的糞便標本910份,採用膠體金法檢測A組輪狀病毒抗原,使用套式反轉錄(RT)-PCR方法對輪狀病毒進行G基因型和P基因型的分型檢測.結果 910份糞便標本中,共檢齣A組輪狀病毒暘性268份,佔29.4％.全年均可檢齣A組輪狀病毒,流行高峰為2008年10月至2009年1月,檢齣率為38.3％～70.5％.＜3歲嬰幼兒的A組輪狀病毒腹瀉有244例,佔91.0％,各年齡組檢齣率以12～23箇月齡組最高,達36.6％.268株A組輪狀病毒中,G基因型以G1最多,為65株(24.3％),以下依次為G3 40株(14.9％)、G混閤型37株(13.8％)、G2 27株(10.1％)、G9 14株(5.2％)、G45株(1.9％),其他G分型5株(1.9％),G未分型75株(28.0％) ;P基因型以P[8]為主,為147株(54.9％),其次為P[4] 32株(11.9％)、P混閤型6株(2.2％),其他P分型4株(1.5％),P未分型79株(29.5％).主要G/P基因型組閤包括G1P[8] (13.4％)、G3P[8](13.4％)、G混閤型P[8](10.1％)、G1P[4](8.2％)、G9P[8](2.2％)、G2P[4](1.9％)、G1P混閤型(1.9％).結論 A組輪狀病毒是上海地區嬰幼兒腹瀉的常見病原體,流行高峰為鞦鼕季節.目前註冊的輪狀病毒疫苗覆蓋瞭上海地區絕大部分輪狀病毒G/P基因型.
목적 감측상해지구복사인동A조륜상병독적분자류행병학,위본지구륜상병독역묘적추엄사용제공배경의거.방법 채집상해지구2008년8월지2009년7월문진급성복사인동적분편표본910빈,채용효체금법검측A조륜상병독항원,사용투식반전록(RT)-PCR방법대륜상병독진행G기인형화P기인형적분형검측.결과 910빈분편표본중,공검출A조륜상병독양성268빈,점29.4％.전년균가검출A조륜상병독,류행고봉위2008년10월지2009년1월,검출솔위38.3％～70.5％.＜3세영유인적A조륜상병독복사유244례,점91.0％,각년령조검출솔이12～23개월령조최고,체36.6％.268주A조륜상병독중,G기인형이G1최다,위65주(24.3％),이하의차위G3 40주(14.9％)、G혼합형37주(13.8％)、G2 27주(10.1％)、G9 14주(5.2％)、G45주(1.9％),기타G분형5주(1.9％),G미분형75주(28.0％) ;P기인형이P[8]위주,위147주(54.9％),기차위P[4] 32주(11.9％)、P혼합형6주(2.2％),기타P분형4주(1.5％),P미분형79주(29.5％).주요G/P기인형조합포괄G1P[8] (13.4％)、G3P[8](13.4％)、G혼합형P[8](10.1％)、G1P[4](8.2％)、G9P[8](2.2％)、G2P[4](1.9％)、G1P혼합형(1.9％).결론 A조륜상병독시상해지구영유인복사적상견병원체,류행고봉위추동계절.목전주책적륜상병독역묘복개료상해지구절대부분륜상병독G/P기인형.
Objective To explore the molecular epidemiological characteristics of group A rotavirus diarrhea in children in Shanghai and to provide the background data for the implementation of rotavirus vaccination.Methods A total of 910 stool samples were collected from the outpatient children with acute diarrhea from August 2008 to July 2009.Group A rotavirus was detected by usingcommercial colloidal gold device.Rotavirus strains were characterized for G and P genotypes using the nested reverse transcription polymerase chain reaction (RT-PCR).Results Group A rotavirus was detected in 268(29.4％) out of 910 stool samples.Rotavirus infection was found year-round and the peak season was from October 2008 to January 2009,with the detection rates ranging from 38.3 ％ to 70.5％.Ninety-one percent of children (244 cases) with rotavirus-associated diarrhea occurred in children ＜3 years of age.The detection rate of rotavirus was highest (36.6％) in children aged 12-23 months.Among the 268 group A rotavirus-positive strains,G1 was the most common G genotype (65 strains),accounting for 24.3％,followed by G3 (40 strains,14.9％),G mixed genotypes (37strains,13.8 ％),G2 (27 strains,10.1％),G9 (14 strains,5.2％),G4(5 strains,1.9％),other G types (5 strains,1.9％),and unclassified G type (75 strains,28.0％).P[8] and P[4] were the most common P genotypes,accounting for 54.9％ (147 strains) and 11.9％ (32 strains),respectively,followed by P mixed genotypes (6 strains,2.2％) and other P genotypes (4 strains,1.5％),unclassified P type (79 strains,29.5％).The G/P genotype combinations were found as follows:G1P [8] (13.4％),G3P[8] (13.4％),GmixP[8] (10.1％),G1P[4] (8.2％),G9P[8] (2.2％),G2P [4] (1.9％),G1Pmix (1.9％).Conclusions Group A rotavirus is a major causative agent of diarrhea in infants and young children in Shanghai.The peak season of rotavirus infection appears in fall and winter.The currently licensed rotavirus vaccines cover the majority of genotypes of rotavirus strains prevailing in Shanghai.
