CAJ | 학술논문

目的观察纤维蛋白凝胶(fibrin glue,FG)复合rhBMP-2及骨髓基质干细胞(mesenchymal stem cells,MSCs)用于兔横突间脊柱融合的效果.方法取1月龄日本大耳白兔股骨MSCs体外原代培养,传代培养时加入成骨诱导物向成骨方向诱导分化.40只1岁龄日本大耳白兔随机分为4组,根据植入的材料分别为:MSCs/rhBMP-2/FG组,MSCs/FG组,rhBMP-2/FG组,FG组.复合物置于皮质已打磨的左侧L5-6横突上.脊柱标本均于术后6周取材,应用手法检测、DR拍片、CT扫描三维重建、灰度分析、生物力学检测、组织学染色等方法观察各组脊柱融合效果.结果手法检测和DR片示MSCs/rhBMP-2/FG组融合率分别为70%和80%,rhBMP-2/FG组经两种方法检测融合率均为40%.MSCs/FG组和FG组无脊柱融合标本.MSCs/rhBMP-2/FG组与rhBMP-2/FG组在融合率上虽无统计学差异,但在成骨密度和面积上存在显著差异,生物力学检测结果示MSCs/rhBMP-2/FG组在各方向上的融合强度均显著高于其他三组.CT扫描三维重建显示MSCs/rhBMP-2/FG组L5-6横突间有连续骨组织生成.组织学染色示MSCs/rhBMP-2/FG组和rhBMP-2/FG组融合标本横突间为成熟骨组织.结论 MSCs/rhBMP-2/FG复合物应用于兔横突间脊柱融合可以取得良好成骨质量.
목적 관찰섬유단백응효(fibrin glue,FG)복합rhBMP-2급골수기질간세포(mesenchymal stem cells,MSCs)용우토횡돌간척주융합적효과.방법 취1월령일본대이백토고골MSCs체외원대배양,전대배양시가입성골유도물향성골방향유도분화.40지1세령일본대이백토수궤분위4조,근거식입적재료분별위:MSCs/rhBMP-2/FG조,MSCs/FG조,rhBMP-2/FG조,FG조.복합물치우피질이타마적좌측L5-6횡돌상.척주표본균우술후6주취재,응용수법검측、DR박편、CT소묘삼유중건、회도분석、생물역학검측、조직학염색등방법관찰각조척주융합효과.결과 수법검측화DR편시MSCs/rhBMP-2/FG조융합솔분별위70%화80%,rhBMP-2/FG조경량충방법검측융합솔균위40%.MSCs/FG조화FG조무척주융합표본.MSCs/rhBMP-2/FG조여rhBMP-2/FG조재융합솔상수무통계학차이,단재성골밀도화면적상존재현저차이,생물역학검측결과시MSCs/rhBMP-2/FG조재각방향상적융합강도균현저고우기타삼조.CT소묘삼유중건현시MSCs/rhBMP-2/FG조L5-6횡돌간유련속골조직생성.조직학염색시MSCs/rhBMP-2/FG조화rhBMP-2/FG조융합표본횡돌간위성숙골조직.결론 MSCs/rhBMP-2/FG복합물응용우토횡돌간척주융합가이취득량호성골질량.
Objective To evaluate the efficacy of MSCs/rhBMP-2/FG composite for posterolateral spinal fusion in a rabbit model.Methods One-month-old rabbit femoral MSCs were separated and cultured.osteogenic inducers was added during subculture.40 rabbits were divided into 4 groups randomly,each according to the material implanfed:MSCs/rhBMP-2/FG,MSCs/FG,rhBMP-2/FG,FG.Each composite was put at the left L5-6 transverse processes.All animals were sacrificed 6 weeks after operation.The specimens were examined by manual palpation,radiography,gray scale analysis,computed tomography,three-dimensional reconstruction,nondestructive biomechanical test and histological examination.Results The fusion ratio of MSCs/rhBMP-2/FG group was 70%and 80%by manual palpation and radiography respectively,while the fusion ratio of was 40%in rhBMP-2/FG group by both above methods.There was no statistical difference in fusion ratio between MSCs/rhBMP-2/FG group and rhBMP-2/FG group.Manual palpation and radiography examination found no fusion in MSCs/FG group and FG group.And gray scale analysis showedthat bone density and holding area of the specimens in MSCs/rhBMP-2/FG group were significantly superior to those in the other three groups.CT scanning and 3D-CT showed there were continuous bone masses between L5-6 transvelse processes in MSCs/rhBMP-2/FG group.Biomechanical test showed fusion strength of the specimens from MSCs/rhBMP-2/FC group were distinctly better than those from other groups.HE stain showed there were mature bone tissues between transverse processes of the fused specimens from MSCs/rhBMP-2/FG and rhBMP-2/FG groups.Conclusion The tissue engineering material composed of MSCs,rhBMP-2 and FG can promote well posterolateral spinal fusion.