中华放射肿瘤学杂志
中華放射腫瘤學雜誌
중화방사종류학잡지
CHINESE JOURNAL OF RADIATION ONCOLOGY
2009年
2期
125-129
,共5页
白守民%梁碧玲%李志伟%韩非%陈春燕%卢泰祥
白守民%樑碧玲%李誌偉%韓非%陳春燕%盧泰祥
백수민%량벽령%리지위%한비%진춘연%로태상
骨髓间充质干细胞%荧光定量%逆转录聚合酶链反应%照射%大鼠
骨髓間充質榦細胞%熒光定量%逆轉錄聚閤酶鏈反應%照射%大鼠
골수간충질간세포%형광정량%역전록취합매련반응%조사%대서
Mesenchymal stem cells%Fluorescent quantitative%Reverse transcriptase polymer-ase chain reaction%Irradiation%Rat
目的 用荧光定量RT-PCR方法探讨骨髓间充质干细胞在大鼠经过全身照射后其体内分布的状况.为进一步研究骨髓间充质干细胞(MSCs)对正常组织器管放射性损伤的治疗提供理论依据.方法 培养MSCs细胞,用绿色荧光蛋白标记.分别对大鼠行全身照射及全身照射+全脑照射,经尾静脉注入标记的MSCs,荧光显微镜观察MSCs在各器官的分布,荧光定量RT-PCR检测MSCs在不同组织器官的分布.结果 获得绿色荧光标记MSCs,经尾静脉注入各实验组大鼠体内后显示,未行照射时MSCs仅少量分布在肺、骨髓、脾中;6 Gy全身照射后各器官MSCs的分布明显增加,小肠、骨髓等早反应组织增加的更显著;7 Gy全身照射进一步增加了各器官MSCs数量;6 Gy全身照射+全脑照射20 Gy后MSCs在大脑的分布明显增多,在其他器官的发布也明显增加.结论 放射损伤可诱导MSCs产生趋化作用;MSCs在各器官分布随照射剂量及放射损伤程度增加而增多;局部照射可增加局部及其他器官MSCs分布.
目的 用熒光定量RT-PCR方法探討骨髓間充質榦細胞在大鼠經過全身照射後其體內分佈的狀況.為進一步研究骨髓間充質榦細胞(MSCs)對正常組織器管放射性損傷的治療提供理論依據.方法 培養MSCs細胞,用綠色熒光蛋白標記.分彆對大鼠行全身照射及全身照射+全腦照射,經尾靜脈註入標記的MSCs,熒光顯微鏡觀察MSCs在各器官的分佈,熒光定量RT-PCR檢測MSCs在不同組織器官的分佈.結果 穫得綠色熒光標記MSCs,經尾靜脈註入各實驗組大鼠體內後顯示,未行照射時MSCs僅少量分佈在肺、骨髓、脾中;6 Gy全身照射後各器官MSCs的分佈明顯增加,小腸、骨髓等早反應組織增加的更顯著;7 Gy全身照射進一步增加瞭各器官MSCs數量;6 Gy全身照射+全腦照射20 Gy後MSCs在大腦的分佈明顯增多,在其他器官的髮佈也明顯增加.結論 放射損傷可誘導MSCs產生趨化作用;MSCs在各器官分佈隨照射劑量及放射損傷程度增加而增多;跼部照射可增加跼部及其他器官MSCs分佈.
목적 용형광정량RT-PCR방법탐토골수간충질간세포재대서경과전신조사후기체내분포적상황.위진일보연구골수간충질간세포(MSCs)대정상조직기관방사성손상적치료제공이론의거.방법 배양MSCs세포,용록색형광단백표기.분별대대서행전신조사급전신조사+전뇌조사,경미정맥주입표기적MSCs,형광현미경관찰MSCs재각기관적분포,형광정량RT-PCR검측MSCs재불동조직기관적분포.결과 획득록색형광표기MSCs,경미정맥주입각실험조대서체내후현시,미행조사시MSCs부소량분포재폐、골수、비중;6 Gy전신조사후각기관MSCs적분포명현증가,소장、골수등조반응조직증가적경현저;7 Gy전신조사진일보증가료각기관MSCs수량;6 Gy전신조사+전뇌조사20 Gy후MSCs재대뇌적분포명현증다,재기타기관적발포야명현증가.결론 방사손상가유도MSCs산생추화작용;MSCs재각기관분포수조사제량급방사손상정도증가이증다;국부조사가증가국부급기타기관MSCs분포.
Objective To detect the distribution of mesenchymal stem cells(MSCs) after total-body irradiation in rats. Methods MSCs were cultured and labeled with green fluorescent protein(GFP). Rats were exposed to total-body irradiation(TB1) or TBI plus total brain irradiation, and then MSCs were injected through the tail vein. The Fluorescent MSCs were observed by fluorescence microscope. The MSCs numbers in different organs were determined by quantitative RT-PCR method. Results GFP-labeled MSCs were ob-tained. After MSCs were infused to the rats,few of them were observed in the organs of nonirradiated group except for a very low number in the lungs,bone marrow(BM) and spleen. TBI of 6 Gy increased the engraft-ment of MSCs in almost all the organs, especially in early response tissues such as the small intestine and BM. TBI of 7 Gy further increased the number of MSCs. The MSCs numbers in the brain and other organs were significantly increased after 20 Gy total brain irradiation in addition to 6 Gy TBI. Conclusions Radi-ation injury can induce the aggregation of MSCs. With the increase of radiation dose and severity of radiation injury,a significant increase of MSCs in different organs were observed. Local irradiation can increase the MSCs distribution in the radiation field as well as other organs.
