中华放射学杂志
中華放射學雜誌
중화방사학잡지
Chinese Journal of Radiology
2008年
9期
978-983
,共6页
沈慧聪%戴建平%魏新华%王建交%李少武%马军%艾林%刘福生%柴奇%赵炜疆%高培毅
瀋慧聰%戴建平%魏新華%王建交%李少武%馬軍%艾林%劉福生%柴奇%趙煒疆%高培毅
침혜총%대건평%위신화%왕건교%리소무%마군%애림%류복생%시기%조위강%고배의
大鼠%神经胶质瘤%磁共振成像,扩散
大鼠%神經膠質瘤%磁共振成像,擴散
대서%신경효질류%자공진성상,확산
Rats%Glioma%Diffusion magnetic resonance imaging
目的 观察常规MR结合扩散加权成像(DWI)在胶质瘤治疗早期疗效的作用.方法 雄性Wistar大鼠50只通过脑立体定向仪于右侧尾状核接种X6胶质瘤细胞10μl(5×105个细胞).于接种后第1周MR检查确认有肿瘤生长后再将其分为对照组和治疗组,其中治疗组大鼠采用上述接种方法于颅内相同位置注入107空斑形成单位的携带血管抑素与内皮抑素融合基因的重组单纯疱疹病毒.分别于接种后第1、2、3周行MR常规及DWI检查,并于每次检查结束后各组分别有2只(第1周)、8只(第2周)及剩余全部大鼠(第3周)安乐死后进行病理检查.对照组与治疗组间不同时间肿瘤体积、表观扩散系数(ADC)值、相同时间不同区域间ADC值的差异比较进行t检验及秩和检验.结果 共43只大鼠见肿瘤生长,成瘤率为86%.对照组与治疗组大鼠C6胶质瘤的体积在第2周时分别为90.60及91.64 mm3,2组间肿瘤体积差异无统计学意义(Z=-0.14,P＞0.05);第3周时2组大鼠肿瘤体积分别为156.64和29.64mm3,两者差异有统计学意义(Z=-3.45,P＜0.01).第2周时治疗组与对照组肿瘤中心的AI)C值分别为(1.20±0.25)×10-3、(0.99±0.08)×10-3 mm2/s,肿瘤边缘的ADC值分别为(1.00±0.25)×10=-3、(0.83±0.12)×10-3 mm2/s,治疗组不同区域的ADC值均高于对照组(t值分别为-4.11,-2.62,P值均＜0.05).第3周时治疗组与对照组肿瘤中心的ADC值分别为(0.92±0.21)×10-3、(0.99±0.09)×10-3 mm2/s,肿瘤边缘的ADC值分别为(0.8l±0.19)×10-3、(0.78±0.11)×10-3 mm2/s,不同区域2组间比较差异无统计学意义(t值分别为0.82,-0.46,P值均＞0.05).结论 DWI能有效地反映大鼠C6脑胶质瘤的组织微观情况;可以早于肿瘤体积发生变化前发现治疗对肿瘤局部细胞状态的影响,从而在肿瘤的疗效观察和预测方面将有更大的发展空间和应用价值.
目的 觀察常規MR結閤擴散加權成像(DWI)在膠質瘤治療早期療效的作用.方法 雄性Wistar大鼠50隻通過腦立體定嚮儀于右側尾狀覈接種X6膠質瘤細胞10μl(5×105箇細胞).于接種後第1週MR檢查確認有腫瘤生長後再將其分為對照組和治療組,其中治療組大鼠採用上述接種方法于顱內相同位置註入107空斑形成單位的攜帶血管抑素與內皮抑素融閤基因的重組單純皰疹病毒.分彆于接種後第1、2、3週行MR常規及DWI檢查,併于每次檢查結束後各組分彆有2隻(第1週)、8隻(第2週)及剩餘全部大鼠(第3週)安樂死後進行病理檢查.對照組與治療組間不同時間腫瘤體積、錶觀擴散繫數(ADC)值、相同時間不同區域間ADC值的差異比較進行t檢驗及秩和檢驗.結果 共43隻大鼠見腫瘤生長,成瘤率為86%.對照組與治療組大鼠C6膠質瘤的體積在第2週時分彆為90.60及91.64 mm3,2組間腫瘤體積差異無統計學意義(Z=-0.14,P＞0.05);第3週時2組大鼠腫瘤體積分彆為156.64和29.64mm3,兩者差異有統計學意義(Z=-3.45,P＜0.01).第2週時治療組與對照組腫瘤中心的AI)C值分彆為(1.20±0.25)×10-3、(0.99±0.08)×10-3 mm2/s,腫瘤邊緣的ADC值分彆為(1.00±0.25)×10=-3、(0.83±0.12)×10-3 mm2/s,治療組不同區域的ADC值均高于對照組(t值分彆為-4.11,-2.62,P值均＜0.05).第3週時治療組與對照組腫瘤中心的ADC值分彆為(0.92±0.21)×10-3、(0.99±0.09)×10-3 mm2/s,腫瘤邊緣的ADC值分彆為(0.8l±0.19)×10-3、(0.78±0.11)×10-3 mm2/s,不同區域2組間比較差異無統計學意義(t值分彆為0.82,-0.46,P值均＞0.05).結論 DWI能有效地反映大鼠C6腦膠質瘤的組織微觀情況;可以早于腫瘤體積髮生變化前髮現治療對腫瘤跼部細胞狀態的影響,從而在腫瘤的療效觀察和預測方麵將有更大的髮展空間和應用價值.
목적 관찰상규MR결합확산가권성상(DWI)재효질류치료조기료효적작용.방법 웅성Wistar대서50지통과뇌입체정향의우우측미상핵접충X6효질류세포10μl(5×105개세포).우접충후제1주MR검사학인유종류생장후재장기분위대조조화치료조,기중치료조대서채용상술접충방법우로내상동위치주입107공반형성단위적휴대혈관억소여내피억소융합기인적중조단순포진병독.분별우접충후제1、2、3주행MR상규급DWI검사,병우매차검사결속후각조분별유2지(제1주)、8지(제2주)급잉여전부대서(제3주)안악사후진행병리검사.대조조여치료조간불동시간종류체적、표관확산계수(ADC)치、상동시간불동구역간ADC치적차이비교진행t검험급질화검험.결과 공43지대서견종류생장,성류솔위86%.대조조여치료조대서C6효질류적체적재제2주시분별위90.60급91.64 mm3,2조간종류체적차이무통계학의의(Z=-0.14,P＞0.05);제3주시2조대서종류체적분별위156.64화29.64mm3,량자차이유통계학의의(Z=-3.45,P＜0.01).제2주시치료조여대조조종류중심적AI)C치분별위(1.20±0.25)×10-3、(0.99±0.08)×10-3 mm2/s,종류변연적ADC치분별위(1.00±0.25)×10=-3、(0.83±0.12)×10-3 mm2/s,치료조불동구역적ADC치균고우대조조(t치분별위-4.11,-2.62,P치균＜0.05).제3주시치료조여대조조종류중심적ADC치분별위(0.92±0.21)×10-3、(0.99±0.09)×10-3 mm2/s,종류변연적ADC치분별위(0.8l±0.19)×10-3、(0.78±0.11)×10-3 mm2/s,불동구역2조간비교차이무통계학의의(t치분별위0.82,-0.46,P치균＞0.05).결론 DWI능유효지반영대서C6뇌효질류적조직미관정황;가이조우종류체적발생변화전발현치료대종류국부세포상태적영향,종이재종류적료효관찰화예측방면장유경대적발전공간화응용개치.
Objective To evaluate the use of diffusion-weighted imaging(DWI)for early detection of tumor response to Angiostatin-Endostatin(Statin-AE)fusion gene therapy in a rat C6 glioma model.Methods Fifty male wistar rats with C6 tumor cells implanted into the striatum were examined by a 3.0T MR scanner,then the rats beating tmors were divided into two groups,treatment group and control group.Rats in the treatment group received 107 plaque forming unit(pfu)recombinant herps simplex viral (R-HSV)mediated Statin-AE fusion gene therapy on day 7,and then the tumors were conformed on MRI.Conventional MR and DWI examination were acquired on 1,2,3 weeks after implantation with a 5-inch surface coil.Two(1 w),eight(2 w)and all the residual rats(3 w)of each group were sacrificed to perform the histopathological examination after each MBI examination.Pretreatment and post treatment tumor volulnes and apparent diffusion coefficient(ADC)values were calculated.Rank sum test and t test were employed for statistical analysis.Results On MRI,43 rats demonstrated tumors on day 7 with a successful rate of 86%,On week 2,the tumor volumes of the controh and treatment group were 90.6 and 91.64 mm3,with no significant difference(Z=-0.14,P＞0.05).On week 3,the tumor volumes of the controls and treatment group were 156.64 and 29.64 mm3,and a significant difference was observed(Z=-3.45,P＜0.01).On week 2.the ADC values of the tumor centers of the treatment group and the control group were (1.20±0.25)×10-3 and(0.99±0.08)×10-3 mm2/s,and the values of the tumor peripheral parts of the two groups were(1.00±0.25)×10-3 and(0.83±0.12)×10-3mm2/s,the ADC values of both tumor centers and peripheral parts of the treatment group were significantly higher than those of the control group (t=-0.82 and-0.46,P＜0.05).On week 3,the ADC values of the tumor centers of the treatment group and the control group were(0.92±0.21)× 10-3 and(0.99±0.09)×10-3mm2/s,and the values of the tumor peripheral parts of the two groups were(0.81±0.19)×10-3 and(0.78±0.11)×10-3 mm2/a,there were no statisfical difference between the two groups(t=0.82,and-0.46,P＜0.05).HE stained slices showed more prominent tumor interstifial edenla.swelling and death of tumor cells in the treated rats than the controls.Conclusions Combination of conventional MRI and DWI can be powerful to monitor tumor progression and therapy effecL Conventional MRI showed that the therapy slow the tumor progression in size while DWI demonstrated the tumor response even earlier than size change.DWI has potential use forthe detection of early response to antiangiogenic gene therapy.
