CAJ | 학술논문

目的研究环氧化酶2(COX-E)的表达与术后粘连发生的关系,探讨选择性COX-E抑制剂塞来昔布在预防术后腹腔粘连中的作用.方法健康SD大鼠50只,随机分为5组(,n=lO),分别为阳性对照组、透明质酸钠组、塞来昔布小剂量组、塞来昔布大剂量组、阴性对照组.每组于造模术后第8天和第15天随机处死大鼠各5只,肉眼观察评估腹腔粘连程度,并取创伤部位肠管及腹膜Western-blot法检测组织COX-2蛋白含量.结果透明质酸钠组、塞来昔布小剂量组、塞来昔布大剂量组腹腔粘连程度以及创伤部位组织COX-2表达明显减低,与阳性对照组相比差异有统计学意义(P<0.05).透明质酸钠组与各塞来昔布组相比差异有统计学意义(P<0.05).结论 COX-2选择性抑制剂塞来昔布通过降低创伤部位组织COX-2表达能减轻术后腹腔粘连.
목적 연구배양화매2(COX-E)적표체여술후점련발생적관계,탐토선택성COX-E억제제새래석포재예방술후복강점련중적작용.방법 건강SD대서50지,수궤분위5조(,n=lO),분별위양성대조조、투명질산납조、새래석포소제량조、새래석포대제량조、음성대조조.매조우조모술후제8천화제15천수궤처사대서각5지,육안관찰평고복강점련정도,병취창상부위장관급복막Western-blot법검측조직COX-2단백함량.결과 투명질산납조、새래석포소제량조、새래석포대제량조복강점련정도이급창상부위조직COX-2표체명현감저,여양성대조조상비차이유통계학의의(P<0.05).투명질산납조여각새래석포조상비차이유통계학의의(P<0.05).결론 COX-2선택성억제제새래석포통과강저창상부위조직COX-2표체능감경술후복강점련.
Objective To study the corelation between the expression of COX-2 and postoperative adhesions and to determine the effect of COX-2 selective inhibitor,Celecoxib,on postoperative adhesion formation. Methods Fifty SD rats were randomly assigned to five groups each consisting of 10 rats,Study groups were as follows:(A)positive control group,(B)sodiumhyahronate group,(C)low dose Celecoxib group,(D)high dose Celecexib group,(E)negative control group,Five rats in each group were treated accordingly for consecutive 8 days and 15 days respectively and sacrificed,After treatment,intra-abdminal adhesions were scored using a standard method.The adhesions tissure and injured peritonaeum were subjected to Westem-blotting to detect the expression of COX-2. Results The level of postoperative adhesions and expression of COX-2 of sodiumhyahronate group、low dose Celecoxib group、high dose Celecoxib group were lower than that of positive control group(P<0.05),Sediumhyahronate treatment group was different formthe two Celecoxib treated groups(P<0.05).Conclusions Selective COX-2COX-2.mechanism,provides durable inhibition of intra-abdominal adhesions through an antiangiongenic COX-2 mechanism.