中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2012年
6期
466-470
,共5页
雷蕙嘉%孙建军%刘阳%李雪盛%彭本刚
雷蕙嘉%孫建軍%劉暘%李雪盛%彭本剛
뢰혜가%손건군%류양%리설성%팽본강
间质干细胞%鼓膜%组织工程%大鼠,Wistar
間質榦細胞%鼓膜%組織工程%大鼠,Wistar
간질간세포%고막%조직공정%대서,Wistar
Mesenchymal stem cells%Tympanic membrane%Tissue engineering%Rats,Wistar
目的 观察大鼠脂肪干细胞(adipose-derived stem cell,ASC)在聚乳酸-羟基乙酸共聚物(polylactic-co-glycolic acid,PLGA)支架上的生长状态、分裂增殖能力及黏附性,为进一步构建组织工程化鼓膜修复材料提供实验参考.方法 以Wistar大鼠为研究对象,分离其ASC,并与PLGA支架材料复合培养.分别行细胞波形蛋白(Vimentin)和Ki67免疫荧光染色,激光共聚焦显微镜下观察ASC在PLGA支架上的生长状态及分裂增殖能力.扫描电镜观察ASC与PLGA的黏附性.结果 Vimentin免疫荧光结果显示ASC在PLGA支架材料中生长状态良好,排列有序；ASC在PLGA支架上能正常活跃增殖,Ki67平均阳性指数((x)±s)为(8.21±1.76)％,对照组(未放支架材料,直接生长的ASC) Ki67平均阳性指数为(9.06±1.95)％,两组间差异无统计学意义(=1.03,P=0.30).扫描电镜结果显示,ASC与支架材料有较好的黏附性,伪足清晰可见,增殖的细胞间连接紧密.结论 大鼠ASC与PLGA有较好的相容性,可以在PLGA上正常黏附、增殖.
目的 觀察大鼠脂肪榦細胞(adipose-derived stem cell,ASC)在聚乳痠-羥基乙痠共聚物(polylactic-co-glycolic acid,PLGA)支架上的生長狀態、分裂增殖能力及黏附性,為進一步構建組織工程化鼓膜脩複材料提供實驗參攷.方法 以Wistar大鼠為研究對象,分離其ASC,併與PLGA支架材料複閤培養.分彆行細胞波形蛋白(Vimentin)和Ki67免疫熒光染色,激光共聚焦顯微鏡下觀察ASC在PLGA支架上的生長狀態及分裂增殖能力.掃描電鏡觀察ASC與PLGA的黏附性.結果 Vimentin免疫熒光結果顯示ASC在PLGA支架材料中生長狀態良好,排列有序；ASC在PLGA支架上能正常活躍增殖,Ki67平均暘性指數((x)±s)為(8.21±1.76)％,對照組(未放支架材料,直接生長的ASC) Ki67平均暘性指數為(9.06±1.95)％,兩組間差異無統計學意義(=1.03,P=0.30).掃描電鏡結果顯示,ASC與支架材料有較好的黏附性,偽足清晰可見,增殖的細胞間連接緊密.結論 大鼠ASC與PLGA有較好的相容性,可以在PLGA上正常黏附、增殖.
목적 관찰대서지방간세포(adipose-derived stem cell,ASC)재취유산-간기을산공취물(polylactic-co-glycolic acid,PLGA)지가상적생장상태、분렬증식능력급점부성,위진일보구건조직공정화고막수복재료제공실험삼고.방법 이Wistar대서위연구대상,분리기ASC,병여PLGA지가재료복합배양.분별행세포파형단백(Vimentin)화Ki67면역형광염색,격광공취초현미경하관찰ASC재PLGA지가상적생장상태급분렬증식능력.소묘전경관찰ASC여PLGA적점부성.결과 Vimentin면역형광결과현시ASC재PLGA지가재료중생장상태량호,배렬유서；ASC재PLGA지가상능정상활약증식,Ki67평균양성지수((x)±s)위(8.21±1.76)％,대조조(미방지가재료,직접생장적ASC) Ki67평균양성지수위(9.06±1.95)％,량조간차이무통계학의의(=1.03,P=0.30).소묘전경결과현시,ASC여지가재료유교호적점부성,위족청석가견,증식적세포간련접긴밀.결론 대서ASC여PLGA유교호적상용성,가이재PLGA상정상점부、증식.
Objective To explore the morphology.,proliferative activity and adhesion of adiposederived stem cells (ASC) seeded in the polylactic-co-glycolic acid (PLGA) scaffold,and to provide experimental data for fabricating tissue engineered tympanic membrane repairment materials.Methods Wistar rats were selected,and the ASC were isolated and co-cultured with the PLGA scaffold. The morphology and proliferative activity of ASC in the scaffold were examined by immunofluorescence of Vimentin and Ki67 respectively.All the immunofluorescence signals were analyzed by a confocal laser scan microscopy system FLUOVIEW FV1000.The adhesion of ASC to the PLGA scalfold was determined by scanning electron microscopy.Results Immunofluorescence of vimentin showed rats ASC displayed normal morphology and grew orderly in the PLGA scalfold.Immunofluorescence of Ki67 showed the normal active proliferation of ASC in the scaffolds.The Ki67 mean positive index ( x ± s) of the ASC in the scalfold was (8.21 ± 1.76 ) ％,while in control group ( cultured without PLGA scalfold ) was ( 9.06 ± 1.95 ) ％.There was no statistic significance between the two groups ( t =1.03,P =0.30 ).Scanning electron microscopy demonstrated ASC adhered well to the PLGA scalfold,the pseudopodia of ASC could also be observed and the proliferative cell conjunction was tight.Conclusion ASC has good biocompatible to the PLGA scaffold and could normally adhere and proliferate in PLGA scaffold.
