CAJ | 학술논문

目的通过对暴露于稀土粉尘工作人员外周血细胞的IL-2、IL-6和TNF-α基因多态性位点的检测,对接尘工作人员的肺通气功能进行初步探讨.方法 TNF-α(-308,G→A)基因多态性检测采用基因体外扩增限制性片段长度多态性分析方法,IL-2(-330,T→G)和IL-6(-174G→C)基因多态性均采用相对的两条引物聚合酶链反应检测.结果接尘组与非接尘组相比较,TNF-α基因多态性(X2=4.03,P＞0.05)、IL-2基因多态性(X2=2.21,P＞0.05)和IL-6基因多态性(X2=1.05,P＞0.05)差异均无统计学意义.IL-2基因突变纯合型致肺通气功能障碍的发病风险是野生型的4.29倍(95%可信限1.09～16.9).结论 IL-2基因突变纯合型更易加重肺部炎症反应.接尘组人员的肺通气功能障碍发生率与非接尘组的相比有增多的趋势.
목적 통과대폭로우희토분진공작인원외주혈세포적IL-2、IL-6화TNF-α기인다태성위점적검측,대접진공작인원적폐통기공능진행초보탐토.방법 TNF-α(-308,G→A)기인다태성검측채용기인체외확증한제성편단장도다태성분석방법,IL-2(-330,T→G)화IL-6(-174G→C)기인다태성균채용상대적량조인물취합매련반응검측.결과 접진조여비접진조상비교,TNF-α기인다태성(X2=4.03,P＞0.05)、IL-2기인다태성(X2=2.21,P＞0.05)화IL-6기인다태성(X2=1.05,P＞0.05)차이균무통계학의의.IL-2기인돌변순합형치폐통기공능장애적발병풍험시야생형적4.29배(95%가신한1.09～16.9).결론 IL-2기인돌변순합형경역가중폐부염증반응.접진조인원적폐통기공능장애발생솔여비접진조적상비유증다적추세.
Objective To study the relationship between lung ventilation function of workers exposed to rare earth dust and their IL-2,IL-6 and TNF-α gene polymorphism.Methods TNF-α gene polymorphism were identified by RFLP-PCR,IL-2 and IL-6 gene polymorphisms were identified by PCR-CTPP analysis.Lung ventilation function was deteced by instrument of ventilation function.Results Compared with controls,there was no statistic significance in frequencies distribution of TNF-α gene polymorphism(X2=4.03,P＞0.05),IL-2 gene polymorphism(X2=2.21,P＞0.05)and IL-6 gene polymorphism(X2=1.05,P＞0.05).Compared with IL-2 gene wild type,IL-2 homozygote type increased the risk of lung ventilation dysfunction by 4.29 folds(95% CI 1.09～16.9).Conclusions Compared with controls,incidence of ventilation function of workers exposed to rare earth dust is in ascending trend.IL-2(G/G)gene type induces more serious inflammation reaction than the others.