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中华医学遗传学杂志 중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2009年 4期 374-378 ,共5页

林克勤%孙浩%张昌军%陶玉芬%易文%黄小琴%史荔%褚嘉祜

림극근%손호%장창군%도옥분%역문%황소금%사려%저가호

EB病毒%环胞霉素A%永生细胞株%伴帕金森氏病症状的脊髓小脑共济性失调2型%微卫星%遗传稳定性 EB病毒%環胞黴素A%永生細胞株%伴帕金森氏病癥狀的脊髓小腦共濟性失調2型%微衛星%遺傳穩定性
EB병독%배포매소A%영생세포주%반파금삼씨병증상적척수소뇌공제성실조2형%미위성%유전은정성
Epstain Barr virus%cyclosporine A%immortal cell lines%spinocerebellar ataxia type 2 with Parkinson disease symptoms%microsatellites%genetic stability

目的建立伴帕金森氏病症状的脊髓小脑共济性失调2型家系永生细胞株,提供永久性的实验研究材料.方法采用EB病毒加入环胞霉素A的转化细胞技术,建立了该家系的永生细胞株.并检测传代细胞与家系血液中的微卫星标记有无改变,确定细胞株的遗传稳定性.结果成功建立此家系25株永生细胞株,所检测的微卫星位点未发生变化.结论所建永生细胞株染色体核型检测无异常,转化细胞株与血液中的微卫星的遗传稳定性无差异.
목적 건립반파금삼씨병증상적척수소뇌공제성실조2형가계영생세포주,제공영구성적실험연구재료.방법 채용EB병독가입배포매소A적전화세포기술,건립료해가계적영생세포주.병검측전대세포여가계혈액중적미위성표기유무개변,학정세포주적유전은정성.결과 성공건립차가계25주영생세포주,소검측적미위성위점미발생변화.결론 소건영생세포주염색체핵형검측무이상,전화세포주여혈액중적미위성적유전은정성무차이.
nes were established successfully from the family and the microsatellite markers were unchanged.Conclusion The karyotypes of the immortal cell lines were normal and the cell lines were genetically stable.