CAJ | 학술논문

目的探讨贲门癌患者的免疫功能异常及其临床意义.方法采用流式细胞术(FCM)和酶联免疫吸附(ELISA)方法分别检测56例贲门癌患者、15名健康人外周血中CD4+CD25hiCD127lowc调节性T细胞(Treg细胞)和血清IL-10、TGF-β1水平,结合临床资料进行分析.结果 56例贲门癌患者外周血中CD4+CD25hiCD127lowTreg细胞占CD4+淋巴细胞的比例为(5.73±1.56)%,与健康对照组的(4.45±1.06)%相比,差异有统计学意义(P<0.01);血清中IL-10和TGF-β1含量均明显高于健康对照(P<0.05).贲门癌患者外周血CD4+CD25hiCD127low Treg细胞水平与血清IL-10和TGF-β1含量呈正相关.贲门癌患者外周血CD4+CD25hiCD127low Treg细胞数量与患者临床分期、淋巴结转移有关.结论贲门癌患者外周血中CD4+CD25hiCD127low Treg细胞表达增高,与临床分期有关,提示Treg水平异常与贲门癌的发生发展密切相关.
목적 탐토분문암환자적면역공능이상급기림상의의.방법 채용류식세포술(FCM)화매련면역흡부(ELISA)방법분별검측56례분문암환자、15명건강인외주혈중CD4+CD25hiCD127lowc조절성T세포(Treg세포)화혈청IL-10、TGF-β1수평,결합림상자료진행분석.결과 56례분문암환자외주혈중CD4+CD25hiCD127lowTreg세포점CD4+림파세포적비례위(5.73±1.56)%,여건강대조조적(4.45±1.06)%상비,차이유통계학의의(P<0.01);혈청중IL-10화TGF-β1함량균명현고우건강대조(P<0.05).분문암환자외주혈CD4+CD25hiCD127low Treg세포수평여혈청IL-10화TGF-β1함량정정상관.분문암환자외주혈CD4+CD25hiCD127low Treg세포수량여환자림상분기、림파결전이유관.결론 분문암환자외주혈중CD4+CD25hiCD127low Treg세포표체증고,여림상분기유관,제시Treg수평이상여분문암적발생발전밀절상관.
Objective To analyse the dysfunction of immunity and clinical significance in patients with cardiac cancer.Methods The level of CD4+ CD25hi CD127low Treg cells were detected by flow cytometry (FCM),and serum IL-10 and TGF-β1 levels were determined by enzyme linked immunosorbent assay (ELISA) kit in 56 patients with cardiac cancer.15 healthy volunteers were tested as normal controls.The clinical data of each patient were collected and analyzed. Results There was a significantly higher percentage of CD4+ CD25hi CD127low Treg cells in patients with cardiac cancer (5.73±1.56)% than that (4.45±1.06)% of healthy volunteers (P<0.01).The IL-10 and TGF-β1 levels in the serum of patients with cardiac cancer were also significantly higher than that of healthy volunteers (P<0.05).There was a positive correlation between levels of IL-10.TGF-β1 and the levels of CD4+ CD25hi CD127low Treg cells.The number of CD4+ CD25hi CD127low regulatory T cells in the peripheral blood of cardiac cancer patients were significantly correlated with clinical stages and metastasis lymph node.Conclusion The CD4+ CD25hi CD127low Treg cells in the peripheral blood of cardiac cancer patients is significantly increased in comparison with that in healthy volunteers,and was also correlated with different stages.The abnormal levels of CD4+ CD25hi CD127low Treg cells may be related to tumor progression in patients with cardiac cancer.