CAJ | 학술논문

背景:海马组织一氧化氮含量和一氧化氮合酶(nitric oxide synthase,NOS)活性异常可能是肥胖发病的重要因素之一,已知针灸减肥机制涉及神经及神经体液调节,针刺对海马组织一氧化氮含量和NOS活性的调整作用如何?目的:探讨针刺对肥胖大鼠海马组织一氧化氮和NOS含量的影响,进一深化针刺减肥的中枢神经作用机制.设计:以自身对照、相互对照实验研究.单位:南京中医药大学第二临床医学院针灸研究所、南京人口管理学院生殖医学教研室.材料:本实验在南京中医药大学第二临床医学院针灸研究所完成.1月龄刚断乳SD雄性大鼠,体质量50～70 g,由南京军区总医院实验动物中心提供[许可证号:SYXK(苏)2002-0017],动物级别为清洁级(合格证号:苏动质字第97001号).干预:以普通全价鼠饲料喂养的大鼠作为正常组,将造模成功的实验性肥胖大鼠随机分为对照组和针刺组,每组6只.针刺组大鼠给予针刺治疗14d,正常组和对照组大鼠分别每天放入大鼠固定器中适应15 min,持续14 d.采用神经生化技术观察针刺治疗前后肥胖大鼠体质量、体脂及海马组织一氧化氮和NOS含量的变化.主要观察指标:①针刺对实验性肥胖大鼠肥胖指标及对心包、肾周和附睾脂肪量的影响.②针刺对各组大鼠海马组织一氧化氮含量和一氧化氮合酶活性的影响.③大鼠海马组织一氧化氮含量和NOS活性与体质量和Lee's指数的相关性.结果:肥胖大鼠体质量[(458.33±7.43)g、Lee's指数(302.23±4.11)和体]脂量均显著高于正常大鼠水平,差异有显著性意义(t=8.86,9.99,8.31,P＜0.01);肥胖大鼠海马组织一氧化氮含量[(0.31±0.08)μmol/g]和NOS活性[(1.67±0.65)μkat/g]也均显著高于正常大鼠水平,差异有显著性意义(t=-6.36,-4.91,P＜0.01).大鼠海马组织一氧化氮含量和NOS活性与体质量和Lee's指数呈高度正相关(r=0.760,0.804).针刺治疗明显降低肥胖大鼠海马组织一氧化氮含量[(0.33±0.10)μmol/g]和NOS活性[(4.17±1.07)μkat/g],与对照组比较,差异有显著性意义(t=5.24,2.88,P＜0.05～0.01).结论:针刺对肥胖机体海马组织一氧化氮含量和NOS的活性有良性调整作用. 揹景:海馬組織一氧化氮含量和一氧化氮閤酶(nitric oxide synthase,NOS)活性異常可能是肥胖髮病的重要因素之一,已知針灸減肥機製涉及神經及神經體液調節,針刺對海馬組織一氧化氮含量和NOS活性的調整作用如何?目的:探討針刺對肥胖大鼠海馬組織一氧化氮和NOS含量的影響,進一深化針刺減肥的中樞神經作用機製.設計:以自身對照、相互對照實驗研究.單位:南京中醫藥大學第二臨床醫學院針灸研究所、南京人口管理學院生殖醫學教研室.材料:本實驗在南京中醫藥大學第二臨床醫學院針灸研究所完成.1月齡剛斷乳SD雄性大鼠,體質量50～70 g,由南京軍區總醫院實驗動物中心提供[許可證號:SYXK(囌)2002-0017],動物級彆為清潔級(閤格證號:囌動質字第97001號).榦預:以普通全價鼠飼料餵養的大鼠作為正常組,將造模成功的實驗性肥胖大鼠隨機分為對照組和針刺組,每組6隻.針刺組大鼠給予針刺治療14d,正常組和對照組大鼠分彆每天放入大鼠固定器中適應15 min,持續14 d.採用神經生化技術觀察針刺治療前後肥胖大鼠體質量、體脂及海馬組織一氧化氮和NOS含量的變化.主要觀察指標:①針刺對實驗性肥胖大鼠肥胖指標及對心包、腎週和附睪脂肪量的影響.②針刺對各組大鼠海馬組織一氧化氮含量和一氧化氮閤酶活性的影響.③大鼠海馬組織一氧化氮含量和NOS活性與體質量和Lee's指數的相關性.結果:肥胖大鼠體質量[(458.33±7.43)g、Lee's指數(302.23±4.11)和體]脂量均顯著高于正常大鼠水平,差異有顯著性意義(t=8.86,9.99,8.31,P＜0.01);肥胖大鼠海馬組織一氧化氮含量[(0.31±0.08)μmol/g]和NOS活性[(1.67±0.65)μkat/g]也均顯著高于正常大鼠水平,差異有顯著性意義(t=-6.36,-4.91,P＜0.01).大鼠海馬組織一氧化氮含量和NOS活性與體質量和Lee's指數呈高度正相關(r=0.760,0.804).針刺治療明顯降低肥胖大鼠海馬組織一氧化氮含量[(0.33±0.10)μmol/g]和NOS活性[(4.17±1.07)μkat/g],與對照組比較,差異有顯著性意義(t=5.24,2.88,P＜0.05～0.01).結論:針刺對肥胖機體海馬組織一氧化氮含量和NOS的活性有良性調整作用.
배경:해마조직일양화담함량화일양화담합매(nitric oxide synthase,NOS)활성이상가능시비반발병적중요인소지일,이지침구감비궤제섭급신경급신경체액조절,침자대해마조직일양화담함량화NOS활성적조정작용여하?목적:탐토침자대비반대서해마조직일양화담화NOS함량적영향,진일심화침자감비적중추신경작용궤제.설계:이자신대조、상호대조실험연구.단위:남경중의약대학제이림상의학원침구연구소、남경인구관이학원생식의학교연실.재료:본실험재남경중의약대학제이림상의학원침구연구소완성.1월령강단유SD웅성대서,체질량50～70 g,유남경군구총의원실험동물중심제공[허가증호:SYXK(소)2002-0017],동물급별위청길급(합격증호:소동질자제97001호).간예:이보통전개서사료위양적대서작위정상조,장조모성공적실험성비반대서수궤분위대조조화침자조,매조6지.침자조대서급여침자치료14d,정상조화대조조대서분별매천방입대서고정기중괄응15 min,지속14 d.채용신경생화기술관찰침자치료전후비반대서체질량、체지급해마조직일양화담화NOS함량적변화.주요관찰지표:①침자대실험성비반대서비반지표급대심포、신주화부고지방량적영향.②침자대각조대서해마조직일양화담함량화일양화담합매활성적영향.③대서해마조직일양화담함량화NOS활성여체질량화Lee's지수적상관성.결과:비반대서체질량[(458.33±7.43)g、Lee's지수(302.23±4.11)화체]지량균현저고우정상대서수평,차이유현저성의의(t=8.86,9.99,8.31,P＜0.01);비반대서해마조직일양화담함량[(0.31±0.08)μmol/g]화NOS활성[(1.67±0.65)μkat/g]야균현저고우정상대서수평,차이유현저성의의(t=-6.36,-4.91,P＜0.01).대서해마조직일양화담함량화NOS활성여체질량화Lee's지수정고도정상관(r=0.760,0.804).침자치료명현강저비반대서해마조직일양화담함량[(0.33±0.10)μmol/g]화NOS활성[(4.17±1.07)μkat/g],여대조조비교,차이유현저성의의(t=5.24,2.88,P＜0.05～0.01).결론:침자대비반궤체해마조직일양화담함량화NOS적활성유량성조정작용.
BACKGROUND: The abnormality of the content of nitrogen monoxide (NO)and activity of nitrogen monoxide synthase(NOS) may be an important factor of obesity. It has been known that the mechanisms of acupuncture treating obesity are related to nervous and neurohumoral regulation. What is the regulating effect of acupuncture on NO content and NOS activity in hippocampal tissues?OBJECTIVE: To study the effects of acupuncture on the content of NO and NOS, and further investigate central nervous functional mechanism of reducing weight by acupuncture.DESIGN: Self-controlled and mutual controlled experiment.SETTING: Acupuncture Institute of Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, and Department of Reproduction Medicine, Nanjing College for Population Programmed Management.PARTICIPANTS: This experiment was carried out in the Acupuncture Institute of Second Clinical Medical College; One-month old male SD rats just in allactation, weighing 50 -70 g were provided by the Animal Experiment Center of General Hospital of Nanjing Military Area Command[Certification No. SYXK(Su) 2002-0017], and the animals were in the clean grade (Certification No. Su Animal Quality 970101 ).INTERVENTIONS: Rats fed with ordinary diet were in the normal group.Experimental obese rats models were randomly divided as control group and acupuncture group, 6 in each. Rats in the acupuncture group were given acupuncture treatment for 14 days, and rats in the normal and control groups were put into rat fixation-machine for 15 minutes, lasting for 14 days. Body mass, body lipid and contents of NO and NOS in hippocampal tissues in obese rats before and after acupuncture were observed with nervous biochemical technology.Effect of acupuncture on NO and NOS contents in hippocampal tissue of and Lee's index in hippocampal tissue of obese rats.RESULTS: The body mass[ (458.33 ± 7.43)g], Lee' s index (302.23± 4. 11 ) and body fat content of obesity were all significantly higher than those in the normal rats( t =8.86, 9.99, 8.31, P ＜ 0.01); NO content [(0.31 ±0.08) μmol/g] and NOS activity [(1.67±0.65) μkat/g] in the hippocampal tissue of obese rats were all markedly higher than those in the normal rats(t= -6.36, -4.91, P ＜ 0.01) .NO content and NOS activity were highly positively correlated with body mass and Lee' s index ( r = 0. 760, 0. 804) . Acupuncture significantly decreased NO content [(0. 33 ±0. 10) μmol/g] and NOS activity[ (4. 17 ± 1.07) μkat/g] in the hippocampal tissue of obese rats as compared with the control group. The differences had significant meaning( t = 5.24, 2. 88, P ＜ 0.05 - 0. 01 ).CONCLUSION: Acupuncture has fairly good regulating effect on NO content and NOS activity in hippocampal tissues of obese rats.