国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2008年
11期
998-999,1002
,共3页
角蛋白19%肺疾病%临床实验室技术
角蛋白19%肺疾病%臨床實驗室技術
각단백19%폐질병%림상실험실기술
Keratin-19%Lung diseases%Clinical laboratory techniques
目的 探讨血清细胞角蛋白19片段(即CYFRA21-1)检测在诊治源于上皮细胞的良性疾病中的意义.方法 采用电化学发光免疫分析技术测定血清及胸腔积液中CYFRA21-1水平,通过生物素-亲和素结合而将KS19.1单克隆抗体-CYFRA21-1-三联二吡啶钌标记单抗BM19.21的夹心式复合物固定在亲和素包被的磁珠上.在三丙胺及电场的作用下发光以测定CYFRA21-1的值.结果 患肺部疾病及并发症组CYFRA21-1水平明显高于单纯肺部疾病组(P<0.01),老年组CYFRA21-1水平明显高于青壮年组(P<0.01).结论 对于患良性肺部疾病伴并发症的老年患者,如果检测出CYFRA21-1水平升高CEA、NSE正常者应进行对症治疗,2周后复检,以鉴别其良恶性.
目的 探討血清細胞角蛋白19片段(即CYFRA21-1)檢測在診治源于上皮細胞的良性疾病中的意義.方法 採用電化學髮光免疫分析技術測定血清及胸腔積液中CYFRA21-1水平,通過生物素-親和素結閤而將KS19.1單剋隆抗體-CYFRA21-1-三聯二吡啶釕標記單抗BM19.21的夾心式複閤物固定在親和素包被的磁珠上.在三丙胺及電場的作用下髮光以測定CYFRA21-1的值.結果 患肺部疾病及併髮癥組CYFRA21-1水平明顯高于單純肺部疾病組(P<0.01),老年組CYFRA21-1水平明顯高于青壯年組(P<0.01).結論 對于患良性肺部疾病伴併髮癥的老年患者,如果檢測齣CYFRA21-1水平升高CEA、NSE正常者應進行對癥治療,2週後複檢,以鑒彆其良噁性.
목적 탐토혈청세포각단백19편단(즉CYFRA21-1)검측재진치원우상피세포적량성질병중적의의.방법 채용전화학발광면역분석기술측정혈청급흉강적액중CYFRA21-1수평,통과생물소-친화소결합이장KS19.1단극륭항체-CYFRA21-1-삼련이필정조표기단항BM19.21적협심식복합물고정재친화소포피적자주상.재삼병알급전장적작용하발광이측정CYFRA21-1적치.결과 환폐부질병급병발증조CYFRA21-1수평명현고우단순폐부질병조(P<0.01),노년조CYFRA21-1수평명현고우청장년조(P<0.01).결론 대우환량성폐부질병반병발증적노년환자,여과검측출CYFRA21-1수평승고CEA、NSE정상자응진행대증치료,2주후복검,이감별기량악성.
Objective To explore the clinical diagnostic value of CYFRA21-1 in benign lung disease deriving from epithelium cells.Methods CYFRA21-1 level in serum and hydrothorax was determined by electrochemiluminescence immunoassay. The complex sandwich (KS 19.1 monoclonal antibody-CYFRA21-1[Ru(bpy)3]2+ labeled monoclonal antibody BMI 9.21) was fixed to the bead enclosed avidin by means of biotin-avidin combination, then the luminescence under the influence of perfluorotripropylamine and electric field luminous was tested for CYFRA21-1.Results The level of CYFRA21-1 in the group suffering from lung disease accompanied by complications was significantly higher than that of single lung disease group (P<0.01). CYFRA21-1 value in the elder group was significantly higher than that of the younger group (P<0.01).Conclusion The gerontal patients suffering from benign lung disease accompanied by complications should be given symptomatic treatment if their CYFRA21-1 level is elevated but CEA and NES was normal. Recheck the above indices two weeks later so as to identify the benign or malignancy of the disease.
