CAJ | 학술논문

目的探讨LBP液基细胞学薄层沉降技术在痰细胞学检查中的应用及肿瘤诊断价值.方法病人从肺部深处用力咳出晨痰至LBP细胞保存基液专用采样瓶内,加2~5 ml 1,4-二硫代苏糖醇化痰液,置振荡器上振荡20～30 min,低速离心机上离心5~10 min,沉渣用缓冲液稀释后入染色舱.结果全部105例标本均与传统涂片方法对比,LBP液基细胞学沉降法阳性率24.7%,传统涂片方法阳性率14.3%,LBP液基细胞学薄层沉降法较传统涂片法高出10.4%.结论 LBP液基细胞学沉降法在痰液中的应用操作简便、成本低、制片非常清晰.对恶性肿瘤的检出率高.
목적 탐토LBP액기세포학박층침강기술재담세포학검사중적응용급종류진단개치.방법 병인종폐부심처용력해출신담지LBP세포보존기액전용채양병내,가2~5 ml 1,4-이류대소당순화담액,치진탕기상진탕20～30 min,저속리심궤상리심5~10 min,침사용완충액희석후입염색창.결과 전부105례표본균여전통도편방법 대비,LBP액기세포학침강법양성솔24.7%,전통도편방법 양성솔14.3%,LBP액기세포학박층침강법교전통도편법고출10.4%.결론 LBP액기세포학침강법재담액중적응용조작간편、성본저、제편비상청석.대악성종류적검출솔고.
Objective This study evaluated the application of sedimentary LBP Liquid based cell preparation in detecting neoplastic cells in sputum. Methods The sputum samples were taken from patient coughing with strength immediately after morning waking up and were preserved in a mixture provided by the manufactory. Each sample was oscillated for 20 min to 30 min after adding 2~5 ml 1,4-DTT. Then the samples were centrifuged for 5 min to 10 min. after adding the diluted buffer, the samples were placed in the stain chamber of the LBP machine. Results One hundred and five cases were recruited in this study. With LBP preparation, the positive rate of detecting malignances was 24.7% against 14.3% of traditional smear preparation. Conclusion Sedimentary LBP cytology preparation is a convenient, inexpensive technology. It improves the possibility to find potentially malignant cells with its clear prepared slides.