CAJ | 학술논문

为了提高马铃薯对真菌病害的抗性,本试验进行了马铃薯转基因抗病育种的初步研究.通过PCR扩增,从生防木霉菌Trichoderma atroviride的cDNA中克隆到一个内切几丁质酶基因Chi,构建了植物表达载体p33ECR-Chi.通过农杆菌介导的转化方法将Chi基因转化到马铃薯栽培品种"费乌瑞它"和"夏波蒂"试管薯片中.经侵染和共培养后,用卡那霉素和羟苄青霉素筛选抗性芽,共获得18株植株,对所得转化植株进行PCR检测,结果12株为阳性,占67%.本试验为利用基因工程技术提高马铃薯的生防能力奠定了基础.
위료제고마령서대진균병해적항성,본시험진행료마령서전기인항병육충적초보연구.통과PCR확증,종생방목매균Trichoderma atroviride적cDNA중극륭도일개내절궤정질매기인Chi,구건료식물표체재체p33ECR-Chi.통과농간균개도적전화방법장Chi기인전화도마령서재배품충"비오서타"화"하파체"시관서편중.경침염화공배양후,용잡나매소화간변청매소사선항성아,공획득18주식주,대소득전화식주진행PCR검측,결과12주위양성,점67%.본시험위이용기인공정기술제고마령서적생방능력전정료기출.
The antagonistic ability of potato against fungal disease was enhanced by acquiring transgenic potato plants via gene engineering. A chitinase gene was cloned via PCR products from cDNA of Trichoderma atroviride. This gene was recombined into the plant expression vector p33ECR and transferred into micro tubers from flasks of two varieties (Favorita and Shepody) of potato via Agrobacterium tumefaciens-mediated transformation. After infection and co-culture, 18 transformed plants were acquired through Kanamycin and Carbenicillin screening tests. After PCR tests for transgenic plants, 67% of the plants (12 plantlets) were positive. This will give support for obtaining high biologically effective potato varieties in future research.