湖南农业科学
湖南農業科學
호남농업과학
HUNAN AGRICULTURAL SCIENCES
2009年
10期
1-3,8
,共4页
周树良%温拥军%徐宁%龚晓%洪亚辉
週樹良%溫擁軍%徐寧%龔曉%洪亞輝
주수량%온옹군%서저%공효%홍아휘
内生真菌%诱变%石杉碱甲
內生真菌%誘變%石杉堿甲
내생진균%유변%석삼감갑
endophytie fungi%mutation%huperzine A
以干层塔内生真菌SHB为出发菌株,采用微波、紫外线、Co_(60)射线、亚硝酸和硫酸二乙酯对内生真菌孢子进行诱变.诱变孢子悬液通过在PDA平板继代培养2次,把单菌落孢子转接至PD培养液中进行发酵培养.利用高效液相色谱检测发酵液,筛选出一株遗传稳定,产石杉碱甲能力较高的高产菌株wB3.其发酵产物中石杉碱甲为原始菌株的3.04倍.
以榦層塔內生真菌SHB為齣髮菌株,採用微波、紫外線、Co_(60)射線、亞硝痠和硫痠二乙酯對內生真菌孢子進行誘變.誘變孢子懸液通過在PDA平闆繼代培養2次,把單菌落孢子轉接至PD培養液中進行髮酵培養.利用高效液相色譜檢測髮酵液,篩選齣一株遺傳穩定,產石杉堿甲能力較高的高產菌株wB3.其髮酵產物中石杉堿甲為原始菌株的3.04倍.
이간층탑내생진균SHB위출발균주,채용미파、자외선、Co_(60)사선、아초산화류산이을지대내생진균포자진행유변.유변포자현액통과재PDA평판계대배양2차,파단균락포자전접지PD배양액중진행발효배양.이용고효액상색보검측발효액,사선출일주유전은정,산석삼감갑능력교고적고산균주wB3.기발효산물중석삼감갑위원시균주적3.04배.
Abtract: The spores of endophytic fungi SHB of Hupezia serrata were mutated by microwave, ultraviolet ray, Co60 ray, nitrous acid and diethyl sulfate. Then, the suspension of mutated spores was sub-cultured twice in PDA plate, and the single colony spores were transplanted to PD medium for ermentation. Finally, a Huperzine A high-producing strain - wB3 with stable inheritance was selected by high erformance liquid chromatography (HPLC). The content of Huperzine A in fermenting product of wB3 is 3.04 times more than the primitive fungi SHB's.
