CAJ | 학술논문

由多主棒孢病菌侵染引起的棒孢霉落叶病是严重危害橡胶生产的一种世界性病害,近年来在中国也有发ⅱ生.在菌龄、细胞壁酶种类、酶作用浓度、作用时间、酶解温度、渗透压稳定剂等因素对橡胶树多主棒孢原生质体制备和再生影响分析的基础上,笔者首次建立了PEG介导的多主棒孢病菌遗传转化体系,并获得了多主棒孢病菌绿色荧光转化子.该体系中,多主棒孢病菌的原生质体再生率为19.12%,转化率为10.34个/μgDNA.
유다주봉포병균침염인기적봉포매락협병시엄중위해상효생산적일충세계성병해,근년래재중국야유발ⅱ생.재균령、세포벽매충류、매작용농도、작용시간、매해온도、삼투압은정제등인소대상효수다주봉포원생질체제비화재생영향분석적기출상,필자수차건립료PEG개도적다주봉포병균유전전화체계,병획득료다주봉포병균록색형광전화자.해체계중,다주봉포병균적원생질체재생솔위19.12%,전화솔위10.34개/μgDNA.
Corynespora leaf fall disease caused by Corynespora cassiicola is a serious disease of rubber plantations worldwide, and has occurred in China recently. The hypha age, types and concentrations of cell wall -degrading enzymes, time and temperatures for digestion, and osmotic pressure stabilizers were determined to observe their effects on formation and regeneration of protoplasts of C. cassiicola. The protoplast transformation system mediated by polyethylene glycol (PEG) was established, and the green fluorescent protein (GFP) transformants was obtained. The regeneration rate of protoplasts was 19.12%, and the transformation rate was 10.34 protoplasts/μgDNA.