CAJ | 학술논문

目的:研究PEG修饰的藻蓝蛋白亚基光敏剂长循环脂质体介导的光动力疗法抗乳腺癌的效果.方法:采用薄膜水合-超声分散法制备PEG修饰的藻蓝蛋白亚基脂质体(PEG-PCS-lip),MTT法检测藻蓝蛋白亚基脂质体对MCF-7(人乳腺癌细胞)、MA-782(鼠乳腺癌细胞)的光动力杀伤效果,流式细胞术(FCM)分析其对肿瘤细胞周期的影响,并对乳腺癌模型鼠做PDT治疗.结果:PEG-PCS-lip介导的PDT作用对乳腺癌细胞有良好的光动力疗效,对MCF-7及MA-782细胞IC_(50)(半数抑制浓度)分别为68 μg/mL、70 μg/mL;FCM的结果显示,当PEG-PCS-lip 浓度为50 μg/mL时,MCF-7凋亡率约为30.5 %; 用10 mg/kg PEG-PCS-lip静脉注射荷瘤小鼠,光照剂量为208.2 J/cm~2时,其抑瘤率可达到72 %;组织切片观察PDT作用后的肿瘤组织,瘤体中间以细胞凋亡为主,瘤体外周以细胞坏死为主,由血管损伤而形成的空腔增多.结论:PEG-PCS-lip在体外对乳腺癌细胞有良好的光动力杀伤效果,肿瘤细胞凋亡及瘤血管破坏是导致肿瘤细胞死亡的主要原因.
목적:연구PEG수식적조람단백아기광민제장순배지질체개도적광동력요법항유선암적효과.방법:채용박막수합-초성분산법제비PEG수식적조람단백아기지질체(PEG-PCS-lip),MTT법검측조람단백아기지질체대MCF-7(인유선암세포)、MA-782(서유선암세포)적광동력살상효과,류식세포술(FCM)분석기대종류세포주기적영향,병대유선암모형서주PDT치료.결과:PEG-PCS-lip개도적PDT작용대유선암세포유량호적광동력료효,대MCF-7급MA-782세포IC_(50)(반수억제농도)분별위68 μg/mL、70 μg/mL;FCM적결과현시,당PEG-PCS-lip 농도위50 μg/mL시,MCF-7조망솔약위30.5 %; 용10 mg/kg PEG-PCS-lip정맥주사하류소서,광조제량위208.2 J/cm~2시,기억류솔가체도72 %;조직절편관찰PDT작용후적종류조직,류체중간이세포조망위주,류체외주이세포배사위주,유혈관손상이형성적공강증다.결론:PEG-PCS-lip재체외대유선암세포유량호적광동력살상효과,종류세포조망급류혈관파배시도치종류세포사망적주요원인.
Objective:To prepare PEG modified Phycocyanin Subunits Liposomes(PEG-PCS-lip)and study its photodynamic therapy(PDT)effects against breast carcinom. Methods: PEG-PCS-lip was prepared by film dispersion method, and photodynamic therapy effect in vitro was assessed with MTT method,cell apotosis was analyzed by flow cytometry (FCM). Then we treated the breast tumor bearing mice with photodynamic therapy. Results: In vitro PDT experiment, the IC_(50) were 68 μg/mL for MCF-7 and 70 μg/mL for MA-782, The apoptosis ratio of MCF-7 reached 30.5 %. In vivo experiment, after injection of 10 mg/kg PEG-PCS-lip in breast tumor bearing mouse, the tumor inhibition ratio was 72 % with the irradiation dose of 208.2 J/cm~2. There was obvious differentiation between outside and inside PDT treated tumor: the outside tissue cells showed cellular necrosis and the inside tissue cells exhibited the nuclear change of apotosis.There were more cavities in the tissue as a result of the occlusion of tumor blood vessels. Conclusion:PEG-PCS-lip keep the biological activity of phycocyanin subunits very well and enhanced tumor inhibition rate in breast carcinoma. The tumor cell death was due to the occlusion of tumor blood vessels and inducement of cell programmed death in nuclei.