CAJ | 학술논문

背景:国外研究证实,波长为470 nm的蓝光对抑制褪黑素分泌,调整生物节律效果最明显.目前国内尚无发光二极管光源用于调节生物节律的实验报道.目的:观察一定强度蓝色发光二极管光源对大鼠视网膜是否存在损伤.设计、时间及地点:随机分组对照动物实验,组织学分析,于2007-05/2008-04在北京大学第三医院动物实验室完成.材料:SD大鼠32只,BN大鼠16只由北京大学第三医院动物部提供.方法:取SD大鼠16只及BN大鼠16只分别以抽签法随机分为实验组与对照组.将实验组大鼠放入光箱中,光源工作参数为:蓝光波长470 nm,光强控制在300～350 μW/cm~2,光照射4 h/d,连续照射3 d.另取SD大鼠16只以抽签法随机实验组(n=8)与对照组(n=8),将实验组大鼠放入光箱中,光源工作参数为:蓝光波长470 nm,光强控制在120~150μW/cm~2,4 h/d,连续照射3 d.对照组均不进行特殊干预.主要观察指标:照射结束后第2天,取大鼠双侧眼球,冰冻切片后苏木精-伊红染色观察各组大鼠视网膜变化.结果:48只大鼠均进入结果分析.强度为300~350μW/cm~2的蓝色发光二极管光源照射时,SD大鼠视网膜变薄,层次不清,细胞排列不整齐;而BN大鼠视网膜结构层次清晰,细胞排列整齐,与对照组比较无明显差异:强度为120~150μW/cm~2 蓝色发光二极管光源照射时,SD大鼠视网膜结构层次清晰,细胞排列整齐,与对照组比较无明显差异.结论;300~350 μW/cm~2蓝光发光二极管光源照射强度对有色素保护的视网膜是安全的,照射强度为120~150 μW/cm~2蓝色发光二极管光源对不同种属大鼠的视网膜都不会造成光损伤.
배경:국외연구증실,파장위470 nm적람광대억제퇴흑소분비,조정생물절률효과최명현.목전국내상무발광이겁관광원용우조절생물절률적실험보도.목적:관찰일정강도람색발광이겁관광원대대서시망막시부존재손상.설계、시간급지점:수궤분조대조동물실험,조직학분석,우2007-05/2008-04재북경대학제삼의원동물실험실완성.재료:SD대서32지,BN대서16지유북경대학제삼의원동물부제공.방법:취SD대서16지급BN대서16지분별이추첨법수궤분위실험조여대조조.장실험조대서방입광상중,광원공작삼수위:람광파장470 nm,광강공제재300～350 μW/cm~2,광조사4 h/d,련속조사3 d.령취SD대서16지이추첨법수궤실험조(n=8)여대조조(n=8),장실험조대서방입광상중,광원공작삼수위:람광파장470 nm,광강공제재120~150μW/cm~2,4 h/d,련속조사3 d.대조조균불진행특수간예.주요관찰지표:조사결속후제2천,취대서쌍측안구,빙동절편후소목정-이홍염색관찰각조대서시망막변화.결과:48지대서균진입결과분석.강도위300~350μW/cm~2적람색발광이겁관광원조사시,SD대서시망막변박,층차불청,세포배렬불정제;이BN대서시망막결구층차청석,세포배렬정제,여대조조비교무명현차이:강도위120~150μW/cm~2 람색발광이겁관광원조사시,SD대서시망막결구층차청석,세포배렬정제,여대조조비교무명현차이.결론;300~350 μW/cm~2람광발광이겁관광원조사강도대유색소보호적시망막시안전적,조사강도위120~150 μW/cm~2람색발광이겁관광원대불동충속대서적시망막도불회조성광손상.
BACKGROUND: Foreign studies have demonstrated that the blue light at 470 nm inhibits melatonin secretion and displays the most obvious biorhythm regulation. To date, light-emitting diode (LED) applied in regulating biorhythm remains poorly explored. OBJECTIVE: To explore whether a certain intensity of LED (blue) light could induce retinal injury in rats. DESIGN, TIME AND SETTING: Randomized, controlled animal experiment was performed at the Animal Laboratory of Peking University Third Hospital between May 2007 and April 2008. MATERIALS: A total of 32 SD rats and 16 BN rats were provided by Animal Department of Peking University Third Hospital. METHODS: A total of 16 SD and 16 BN rats were respectively randomly divided into test and control groups. Test group rats were placed in light boxes which were controlled by blue LED (wavelength 470 nm) at a intensity of 300-350μW/cm2, 4 hours everyday for 3 days. The remaining SD rats were placed in light box which was controlled by blue LED (wavelength 470 nm) at a intensity of 120-150μW/cm2, 4 hours everyday for 3 days. The control rats were not treated. MAIN OUTCOME MEASURES: At the second day after light irradiation, the rats of all groups were sacrificed and both eyeballs were harvested. The frozen sections were subjected to hematoxylin-eosin staining to observe changes of rat retina. RESULTS: A total of 48 rats were included in final analysis. The retina of SD rats became thinning and disorderly arranged following blue LED irradiation at density of 300-350μW/cm2, but the retina of BN rat remained unchanged similar to control group. After blue LED irradiation at density of 120-150μW/cm2, the retina of SD rat remained unchanged similar to control group. CONCLUSION: Blue LED light source irradiation at a intensity of 300-350μW/cm2 is safe to pigment-protected retina, and at a intensity of 120-150μW/cm2 does not injury retina of different races of rats.