科技导报
科技導報
과기도보
SCIENCE & TECHNOLOGY REVIEW
2010年
4期
21-27
,共7页
丁叶%普天春%余锐萍%张成林%闫鹤%尹君%田纪景%张金国%李睿文%李文贵
丁葉%普天春%餘銳萍%張成林%閆鶴%尹君%田紀景%張金國%李睿文%李文貴
정협%보천춘%여예평%장성림%염학%윤군%전기경%장금국%리예문%리문귀
大熊猫%肝脏%肾脏%器官病理学%肝炎
大熊貓%肝髒%腎髒%器官病理學%肝炎
대웅묘%간장%신장%기관병이학%간염
panda%liver%kidney%organpathology%hepatitis
为了解发病死亡的大熊猫肝脏、肾脏的器官病理学变化特点,本研究采用组织病理学、组织化学和免疫组织化学等方法,对9例发病死亡的大熊猫肝脏和肾脏的组织病理形态学变化进行了系统观察.同时检测观察HBV和HEV抗原在大熊猫肝脏、肾脏组织内的分布定位.组织病理学观察发现,9只大熊猫的肝脏和肾脏均表现出不同程度的病变.肝脏主要表现为淤血、水肿,严重的有出血变化.肝细胞普遍变性,散在单个性坏死或多发性局灶性坏死,个别出现大片坏死、淋巴细胞等炎性细胞浸润、汇管区胆管增生、肝细胞萎缩、纤维结缔组织增生、含铁血黄素沉着等变化.肾脏主要表现为肾球囊扩张,肾小管上皮细胞出现不同程度的变性坏死,肾小管内出现蛋白管型,间质程度不同的纤维化和程度不同的炎性细胞浸润.免疫组织化学观察结果发现,9只大熊猫HBV和HEV的阳性检出率比较高,但各例肝肾组织内免疫组织化学阳性反应信号的强弱不尽相同.Mallory三色染色结果显示,9只大熊猫的肝脏和肾脏均出现不同程度的纤维组织增生.上述结果表明,大熊猫的肝脏和肾脏普遍存在炎症病变,而且大多都有不同程度的纤维组织增生性病变.这些病变的发生可能与肝炎病毒的感染有一定的关系.
為瞭解髮病死亡的大熊貓肝髒、腎髒的器官病理學變化特點,本研究採用組織病理學、組織化學和免疫組織化學等方法,對9例髮病死亡的大熊貓肝髒和腎髒的組織病理形態學變化進行瞭繫統觀察.同時檢測觀察HBV和HEV抗原在大熊貓肝髒、腎髒組織內的分佈定位.組織病理學觀察髮現,9隻大熊貓的肝髒和腎髒均錶現齣不同程度的病變.肝髒主要錶現為淤血、水腫,嚴重的有齣血變化.肝細胞普遍變性,散在單箇性壞死或多髮性跼竈性壞死,箇彆齣現大片壞死、淋巴細胞等炎性細胞浸潤、彙管區膽管增生、肝細胞萎縮、纖維結締組織增生、含鐵血黃素沉著等變化.腎髒主要錶現為腎毬囊擴張,腎小管上皮細胞齣現不同程度的變性壞死,腎小管內齣現蛋白管型,間質程度不同的纖維化和程度不同的炎性細胞浸潤.免疫組織化學觀察結果髮現,9隻大熊貓HBV和HEV的暘性檢齣率比較高,但各例肝腎組織內免疫組織化學暘性反應信號的彊弱不儘相同.Mallory三色染色結果顯示,9隻大熊貓的肝髒和腎髒均齣現不同程度的纖維組織增生.上述結果錶明,大熊貓的肝髒和腎髒普遍存在炎癥病變,而且大多都有不同程度的纖維組織增生性病變.這些病變的髮生可能與肝炎病毒的感染有一定的關繫.
위료해발병사망적대웅묘간장、신장적기관병이학변화특점,본연구채용조직병이학、조직화학화면역조직화학등방법,대9례발병사망적대웅묘간장화신장적조직병리형태학변화진행료계통관찰.동시검측관찰HBV화HEV항원재대웅묘간장、신장조직내적분포정위.조직병이학관찰발현,9지대웅묘적간장화신장균표현출불동정도적병변.간장주요표현위어혈、수종,엄중적유출혈변화.간세포보편변성,산재단개성배사혹다발성국조성배사,개별출현대편배사、림파세포등염성세포침윤、회관구담관증생、간세포위축、섬유결체조직증생、함철혈황소침착등변화.신장주요표현위신구낭확장,신소관상피세포출현불동정도적변성배사,신소관내출현단백관형,간질정도불동적섬유화화정도불동적염성세포침윤.면역조직화학관찰결과발현,9지대웅묘HBV화HEV적양성검출솔비교고,단각례간신조직내면역조직화학양성반응신호적강약불진상동.Mallory삼색염색결과현시,9지대웅묘적간장화신장균출현불동정도적섬유조직증생.상술결과표명,대웅묘적간장화신장보편존재염증병변,이차대다도유불동정도적섬유조직증생성병변.저사병변적발생가능여간염병독적감염유일정적관계.
The samples of nine cases of panda liver and kidney were collected from the veterinary hospital of Beijing Zoo.The histopathological method was used to observe the pathological lesion of the liver and kidney tissues.The cytochemistry and immunohistochemistric methods were used for detection of the hepatitis virus infection in pandas.The results show that,there are varying degrees of pathological changes in liver and kidney tissues in all samples.The livers see extensive congestion,edema and hemorrhage,and widespread degeneration and atrophy of hepatocytes are observed in solne cases.Apart from sporadic necrosis,hepatocytes show multiplefocal necrosis and widespread necrosis.Bile duct proliferation is present in the portal area.The liver interstitium shows inflammatory cell infiltration and fibroplasia.Hemosiderin pigmentation is found in the liver in all cases.Kidney pathological changes consist of extension of the renal glomerulus capsular space,degeneration and necrosis of renal tubular epithelial cells,fibrosis and inflammatory cell infiltration of renal interstitium,and protein cast in the renal tubular.Immunohistochemistry shows that both the positive detectable rates of HBV and HEV are high,however,the extents of the positive reaction are obviously different.Mallory trichromatic staining shows that different levels of proliferation of the fibrous tissue are found in the liver and kidney tissues in the nine cages of pandas.These studies provide evidence that the inflammation reaction prevails in the liver and kidney tissues in the nine eases of pandas and the initiation of the inflammation may be closely related to HBV and HEV.