CAJ | 학술논문

本试验应用免疫组织化学方法和显微图像分析技术,研究了2、16、30、44、58日龄岭南黄鸡外周器官中Ghrelin免疫反应阳性细胞的定位分布与发育性变化.结果表明,在鸡腺胃、肠、心、肝、脾、肺、肾、胰、脑垂体、肾上腺、胸腺、法氏囊中均可观察到Ghrelin免疫阳性反应.其阳性细胞类型包括:腺胃腺小管的内分泌细胞,肠道黏膜上皮和肠腺内的内分泌细胞,肠道黏膜下层和肌层间的神经丛,心内膜下层的蒲肯野纤维,肝血窦中的枯否氏细胞,脾的巨噬细胞和网状细胞,肺的巨噬细胞,肾脏肾小囊壁层的扁平上皮细胞和脏层的足细胞、球内系膜细胞和球旁复合体,胰腺的胰岛细胞,腺垂体的部分嗜酸性细胞和嗜碱性细胞,肾上腺的嗜铬细胞,胸腺上皮细胞、胸腺小体和巨噬细胞,法氏囊黏膜上皮和小结相关上皮内的内分泌细胞、囊小结的网状细胞、巨噬细胞、皮质髓质交界处的上皮细胞等.2～30日龄鸡,随着日龄的增长,各器官中的Ghrelin表达量增加,44、58日龄则有所下降.
본시험응용면역조직화학방법화현미도상분석기술,연구료2、16、30、44、58일령령남황계외주기관중Ghrelin면역반응양성세포적정위분포여발육성변화.결과표명,재계선위、장、심、간、비、폐、신、이、뇌수체、신상선、흉선、법씨낭중균가관찰도Ghrelin면역양성반응.기양성세포류형포괄:선위선소관적내분비세포,장도점막상피화장선내적내분비세포,장도점막하층화기층간적신경총,심내막하층적포긍야섬유,간혈두중적고부씨세포,비적거서세포화망상세포,폐적거서세포,신장신소낭벽층적편평상피세포화장층적족세포、구내계막세포화구방복합체,이선적이도세포,선수체적부분기산성세포화기감성세포,신상선적기락세포,흉선상피세포、흉선소체화거서세포,법씨낭점막상피화소결상관상피내적내분비세포、낭소결적망상세포、거서세포、피질수질교계처적상피세포등.2～30일령계,수착일령적증장,각기관중적Ghrelin표체량증가,44、58일령칙유소하강.
In order to investigate the distribution of ghrelin-immunopositive cells in chickens,some samples of broiler chickens aged 2,16,30,44 and 58 d were collected and studied by immunohistochemistry and microscopic image analysis.The ghrelin-immunopositive reaction was found in the glandular stomach,intestine,heart,liver,spleen,lung,kidney,pancreas,pituitary,adrenal gland,thymus,and bursa of Fabricius.The types of ghrelin-immunopositive cells (ghrelinic) were as follows: some endocrine cells and nerve plexes in the glandular stomach and intestine;Purkinje fibers in heart;Kupffer's cells in liver;macrophages and reticular cells in spleen;pulmonary macrophages;podocytes,Intraglomerular mesangial cells,Juxtaglomerular cells in kidney;pancreatic islets cells in pancreas;some acidophilic cells and basophilic cells in pituitary;chromaffin cells in adrenal gland;thymic epithelial cells,thymic corpuscles and macrophages in thymus;some endocrine cells,reticular cells,macrophages and epithelial cells in bursa of Fabricius.With the chicken growth,the expressive contents of ghrelin were increased from aged 2 to 30 d,but slightly decreased at aged 44 and 58 d in various organs of chickens.