CAJ | 학술논문

目的观察中药丹皮酚对胶原诱导性关节炎(CIA)的治疗作用,并初步探讨其作用机制.方法以胶原诱导法构建关节炎小鼠模型,将成功造模的小鼠随机分为磷酸盐缓冲液(PBS)阴性对照组、甲氨蝶呤(MTX)阳性对照组和丹皮酚组,分别给予相应处理,观察丹皮酚对CIA的临床评分、血清细胞因子、Th17及自身抗体滴度的影响.结果丹皮酚能明显降低关节炎分值(2.36±044,P<0.01),抑制Th1型炎性细胞因子白细胞介素(IL)-12(463±56,P<0.05)、肿瘤坏死因子(TNF)-α(248±66,P<0.01)、干扰素(IFN)-γ(325±40,P<0.05)、IL-6(282±43,P<0.01)等的产生,促进Th2型细胞因子IL-4(1411±683,P<0.01)和IL-10(325±35,P<0.05)分泌,下调脾脏细胞中Th17丰度(1.73%,P<0.05),降低血清抗Ⅱ型胶原自身抗体含量(A值=0.064,P<0.01).结论丹皮酚对CIA具有良好的治疗效果,为研发治疗人类类风湿关节炎药物提供一条新思路.
목적 관찰중약단피분대효원유도성관절염(CIA)적치료작용,병초보탐토기작용궤제.방법 이효원유도법구건관절염소서모형,장성공조모적소서수궤분위린산염완충액(PBS)음성대조조、갑안접령(MTX)양성대조조화단피분조,분별급여상응처리,관찰단피분대CIA적림상평분、혈청세포인자、Th17급자신항체적도적영향.결과 단피분능명현강저관절염분치(2.36±044,P<0.01),억제Th1형염성세포인자백세포개소(IL)-12(463±56,P<0.05)、종류배사인자(TNF)-α(248±66,P<0.01)、간우소(IFN)-γ(325±40,P<0.05)、IL-6(282±43,P<0.01)등적산생,촉진Th2형세포인자IL-4(1411±683,P<0.01)화IL-10(325±35,P<0.05)분비,하조비장세포중Th17봉도(1.73%,P<0.05),강저혈청항Ⅱ형효원자신항체함량(A치=0.064,P<0.01).결론 단피분대CIA구유량호적치료효과,위연발치료인류류풍습관절염약물제공일조신사로.
Objective To observe the effects of paeonol on collagen induced arthritis (CIA) and investigate its mechanisms. Methods The tentative arthritis mice model was created by injecting type Ⅱ collagen. The severity degree of RA was scored according to the rating criteria after treatment with paeonol. ELISA method was employed to detect the levels of IL-12, TNF-2, IFN-γ and anti-type Ⅱ collagen autoantibody. The efficacy of paeonol was compared with that of MTX (the positive control) and PBS was used as the negative control. Results We found that paeonol could alleviate severe RA, inhibit the expression of inflammatory cytokines such as IL-12, TNF-2, IFN-γ and reduce the level of anti-type Ⅱ collagen autoantibody in serum, which was similar with MTX. Conclusion The results show that paeonol has a reliable therapeutic effect for RA. This study has provided a good clue for the future research and development of medications for the treatment of RA.