CAJ | 학술논문

目的探讨氢质子磁共振波谱分析(1H-MRS)结合神经病理手段对急性热应激(HS)和热惊厥(FC)后脑神经元代谢和损伤的早期检测价值.方法采用热水浴诱导21 d龄大鼠FC模型,应用1H-MRS检测HS和FC后脑内N-乙酰天门冬氨酸(NAA)、复合胆碱(Cho)、肌酸(Cr)和乳酸(Lac)含量的变化,原位杂交检测海马锌离子转运体3(ZnT3)mRNA表达.结果 MRS检测结果显示对照组、HS组与FC组NAA/Cr比值分别为1.50±0.42,1.57±0.50和1.61±0.37,Cho/Cr比值分别为0.93±0.27,1.13±0.17和1.28±0.31,各组间差异均无统计学意义(P0.05);Lac/Cr仅见于FC组,为0.64±0.23.FC组海马齿状回检测到ZnT3 mRNA表达.结论 Lac波峰是惊厥性神经元损伤的特征性1H-MRS变化;ZnT3与海马苔藓纤维再生性发芽密切相关;单次短暂的FC发作也能使脑神经元早期发生不同于HS的显著的神经活性物质表达和物质代谢的变化,对脑神经元造成一定的损伤.
목적 탐토경질자자공진파보분석(1H-MRS)결합신경병리수단대급성열응격(HS)화열량궐(FC)후뇌신경원대사화손상적조기검측개치.방법 채용열수욕유도21 d령대서FC모형,응용1H-MRS검측HS화FC후뇌내N-을선천문동안산(NAA)、복합담감(Cho)、기산(Cr)화유산(Lac)함량적변화,원위잡교검측해마자리자전운체3(ZnT3)mRNA표체.결과 MRS검측결과현시대조조、HS조여FC조NAA/Cr비치분별위1.50±0.42,1.57±0.50화1.61±0.37,Cho/Cr비치분별위0.93±0.27,1.13±0.17화1.28±0.31,각조간차이균무통계학의의(P0.05);Lac/Cr부견우FC조,위0.64±0.23.FC조해마치상회검측도ZnT3 mRNA표체.결론 Lac파봉시량궐성신경원손상적특정성1H-MRS변화;ZnT3여해마태선섬유재생성발아밀절상관;단차단잠적FC발작야능사뇌신경원조기발생불동우HS적현저적신경활성물질표체화물질대사적변화,대뇌신경원조성일정적손상.
Objective To asses the value of proton magnetic resonance spectroscopy (1H-MRS) combined with neuropathological findings in early detection of metabolic abnormities and damages of the brain neurons following heat stress (HS) and febrile convulsion (FC). Methods Febrile convulsion models were established in weaning rats (21 days old) by means of hot water bath. -1H-MRS was performed to measure the changes in N-acetylaspartate (NAA), choline (cho), lactate (Lac) and creatine (Cr) contents in the brain tissue following HS or FC, and in sire hybridization was used to detect Zinc transporter 3 (ZnT3) mRNA expression in the hippocampus. Results In the control group, HS group and FC group, the NAA/Cr ratio (1.5±0.42, 1.57±0.59, and 1.61±0.37, respectively) and Cho/Cr ratios showed no significant differences, but a significant increase in Lac/Cr ratio was observed in FC group. ZnT3 3 mRNA expression was detected in the dentate gyms of the rats following the onset of FC. Conclusions As Lac increase is a putative marker of seizure-induced neuronal damage, and ZnT3 is associated with mossy fiber sprouting in the hippocampus, our results suggest that even a single temporary FC may result in marked changes in neuronal metabolism and cause subtle brain injury.