中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
32期
2299-2301
,共3页
Zhenghao%蔡松晏%黄广建%武春涛%GAN Jun%胡承恩%NI Quan-xing%张延龄
Zhenghao%蔡鬆晏%黃廣建%武春濤%GAN Jun%鬍承恩%NI Quan-xing%張延齡
Zhenghao%채송안%황엄건%무춘도%GAN Jun%호승은%NI Quan-xing%장연령
肿瘤治疗方案%炭%阿霉素%纳米%淋巴化疗
腫瘤治療方案%炭%阿黴素%納米%淋巴化療
종류치료방안%탄%아매소%납미%림파화료
Antineoplastic protocols%Charcoal%Doxorubicin%Nanometer%Intralymphatic chemotherapy
目的 应用纳米活性炭粒吸附阿霉素开展淋巴化疗,观察其在犬体内的药代动力学变化.方法 活性炭混悬液2 ml与阿霉素5 mg混匀后注射于犬胃窦前壁浆膜下,质谱仪测定不同时间点的胃网膜右动脉旁淋巴结、胸导管内淋巴液及血液内阿霉素浓度.结果 胃窦前壁浆膜下注射活性炭吸附的阿霉素,立即见注射点周围出现黑色的细淋巴管及黑染的淋巴结,犬胃网膜右动脉旁淋巴结内阿霉素含量在第30分钟时已升高,在第60分钟时达高峰[(84.6±2.0) μg/g组织],持续72 h后仍维持于较高水平;犬胸导管内淋巴液阿霉素浓度在第30分钟时达高峰(162.5 ng/ml),以后持续缓慢下降,在第6小时时仍可测出淋巴液内有阿霉素的存在;血液中未测出阿霉素的痕迹.结论 应用纳米活性炭粒吸附阿霉素开展淋巴化疗,引流淋巴液、淋巴结内阿霉素含量高、维持时间长.
目的 應用納米活性炭粒吸附阿黴素開展淋巴化療,觀察其在犬體內的藥代動力學變化.方法 活性炭混懸液2 ml與阿黴素5 mg混勻後註射于犬胃竇前壁漿膜下,質譜儀測定不同時間點的胃網膜右動脈徬淋巴結、胸導管內淋巴液及血液內阿黴素濃度.結果 胃竇前壁漿膜下註射活性炭吸附的阿黴素,立即見註射點週圍齣現黑色的細淋巴管及黑染的淋巴結,犬胃網膜右動脈徬淋巴結內阿黴素含量在第30分鐘時已升高,在第60分鐘時達高峰[(84.6±2.0) μg/g組織],持續72 h後仍維持于較高水平;犬胸導管內淋巴液阿黴素濃度在第30分鐘時達高峰(162.5 ng/ml),以後持續緩慢下降,在第6小時時仍可測齣淋巴液內有阿黴素的存在;血液中未測齣阿黴素的痕跡.結論 應用納米活性炭粒吸附阿黴素開展淋巴化療,引流淋巴液、淋巴結內阿黴素含量高、維持時間長.
목적 응용납미활성탄립흡부아매소개전림파화료,관찰기재견체내적약대동역학변화.방법 활성탄혼현액2 ml여아매소5 mg혼균후주사우견위두전벽장막하,질보의측정불동시간점적위망막우동맥방림파결、흉도관내림파액급혈액내아매소농도.결과 위두전벽장막하주사활성탄흡부적아매소,립즉견주사점주위출현흑색적세림파관급흑염적림파결,견위망막우동맥방림파결내아매소함량재제30분종시이승고,재제60분종시체고봉[(84.6±2.0) μg/g조직],지속72 h후잉유지우교고수평;견흉도관내림파액아매소농도재제30분종시체고봉(162.5 ng/ml),이후지속완만하강,재제6소시시잉가측출림파액내유아매소적존재;혈액중미측출아매소적흔적.결론 응용납미활성탄립흡부아매소개전림파화료,인류림파액、림파결내아매소함량고、유지시간장.
Objective To observe the pharmacokinetics of adriamycin-adsorbing nanometric activated carbon in intralymphatic chemotherapy. Methods Two ml of suspension of activated carbon with the diameter of 21 nm was mixed with adriamycin 5 mg. Eighteen dogs were randomly divided into 6 equal groups. The above mentioned mixture was injected subserosally to the anterior wall of gastric antrum of the dogs. Thirty minutes, 1 h, 2 h, 1 day, and 3 days after the injection the gastruepiploic lymph nodes of the Groups 1 -5 were obtained. And Group 6 underwent extraction of venous blood samples 5, 15, 30, 60, 120, and 240 minutes after the injection and extraction of thoracic duct fluid 5, 15, 30, 60, 120,240, and 360 minutes after the injection. The adriamycin concentrations at different time points were determined by mass spectrometer. The lymphatic vessels and nodes at the gastric wall were observed by the naked eyes. Results Black tiny lymphatic vessels and lymph nodes were visualized around the injection areas immediately after the injection. Adriamycin content could be detected 30 min after the injection and lasted for 72 h at high levels with the peak content of (84. 6 ± 2.0) μg per gram tissue at 60 min in the paralymph node of gastroepiploic artery. The adriamycin concentration in the lymph fluid of thoracic duct reached the top level of 162. 5 ng/ml 30 min after the injection, and then decreased slowly. Adriamycin could be still detected in lymph fluid 6 h after injection. No trace of adriamycin was found in the blood at any time points. Conclusion The content of adriamycin can keep high and last long in the drainage of lymph node and lymph fluid in the treatment of intralymphatie chemotherapy using adriamycin-adsorbing nanometric activated carbon.
