东南大学学报(英文版)
東南大學學報(英文版)
동남대학학보(영문판)
JOURNAL OF SOUTHEAST UNIVERSITY
2009年
1期
128-131
,共4页
段艳冰%余佳%刘海峰%吉民
段豔冰%餘佳%劉海峰%吉民
단염빙%여가%류해봉%길민
高效液相色谱法%定量分析%蒽醌类前药%活性化合物
高效液相色譜法%定量分析%蒽醌類前藥%活性化閤物
고효액상색보법%정량분석%은곤류전약%활성화합물
high·performance liquid chromatography(HPLC)%quantitative analysis%anthraquinone prodrug%acUve components
为了研究一个结构全新具有潜在抗炎活性蒽醌类前药的体外水解活性,利用反向高效液相色谱法(RP-HPLC)建立了专属而灵敏的同时测定抗炎前药及其活性水解产物的方法.色谱条件如下:ODS-2 C18柱(250 mm×4.6mm,5.0μm填料),流动相V(甲醇):V(O.1%磷酸水溶液)=90:10(调节pH=2.3),检测波长225nm,流速1.0 mL/min.在此条件下,各化合物到达基线分离,3个化合物的拟合标准曲线的R2均大于0.999.该方法同时测定了前药及其活性代谢产物,可以用于前药体外水解的研究.
為瞭研究一箇結構全新具有潛在抗炎活性蒽醌類前藥的體外水解活性,利用反嚮高效液相色譜法(RP-HPLC)建立瞭專屬而靈敏的同時測定抗炎前藥及其活性水解產物的方法.色譜條件如下:ODS-2 C18柱(250 mm×4.6mm,5.0μm填料),流動相V(甲醇):V(O.1%燐痠水溶液)=90:10(調節pH=2.3),檢測波長225nm,流速1.0 mL/min.在此條件下,各化閤物到達基線分離,3箇化閤物的擬閤標準麯線的R2均大于0.999.該方法同時測定瞭前藥及其活性代謝產物,可以用于前藥體外水解的研究.
위료연구일개결구전신구유잠재항염활성은곤류전약적체외수해활성,이용반향고효액상색보법(RP-HPLC)건립료전속이령민적동시측정항염전약급기활성수해산물적방법.색보조건여하:ODS-2 C18주(250 mm×4.6mm,5.0μm전료),류동상V(갑순):V(O.1%린산수용액)=90:10(조절pH=2.3),검측파장225nm,류속1.0 mL/min.재차조건하,각화합물도체기선분리,3개화합물적의합표준곡선적R2균대우0.999.해방법동시측정료전약급기활성대사산물,가이용우전약체외수해적연구.
In order to study the hydrolytic characterization of an and-inflammatory prodrug(RI-1) m vitro,an effective,accurate and reliable method for the simultaneous determination of the prodrug and itS two hydrolytic active compounds is developed using reverse phase high-performance liquid chromatography (RP-HPLC).The chromatographic separation is performed on an ODS-2 C18,column(250 mm×4.6 mm,5.0 μm particle size)with a simple elution program.The mobile phase is V(methanol):V(0.1%phosphoric acid solution)=90:10(adjust pH to 2.3).A wavelength of 225 nm and a mobile phase flow rate of 1.0mL/min are utilized for the quantitative analysis.Excellent linear behaviors over the investigated concentration ranges are observed with values of R2 higher than 0.999 for all the analytes.The validated method is successfully applied to the simultaneous determination of the prodrug and its active components can be used to detect hydrolytic characterization in vitro.