物理化学学报
物理化學學報
물이화학학보
ACTA PHYSICO-CHIMICA SINICA
2011年
1期
213-227
,共15页
维生素E%磷脂%取向%翻转%扩散%氢键
維生素E%燐脂%取嚮%翻轉%擴散%氫鍵
유생소E%린지%취향%번전%확산%경건
Vitamin E%Phospholipid%Orientation%Flip-flop%Diffusion%Hydrogen-bond
用分子动力学方法模拟了280,310和350 K-卜a-生育酚在二豆蔻酞磷脂酞胆碱、二豆蔻酞磷脂酞乙醇胺、二硬脂酞磷脂酞胆碱和二硬脂酞磷脂酞乙醇胺双层膜中的性质,包括了空间位置、氢键、取向和动力学性质,取得了如下的结论.第一,生育酚头部的羟基一般位于脂双层亲疏水界面的下方,升高温度将促进羟基向膜双层的中心移动,在350K时观察到了在上下两个单层间的翻转.第二,生育酚主要与磷脂的酯基形成氢键,几乎不与磷脂酞乙醇胺的氨基形成氢键;比较生育酚与磷脂酞胆碱和乙醇胺形成的氢键后发现,后者更稳定.第三,生育酚的头部在膜中取向多变,与膜的法线夹角不固定,尾部的构象也很复杂.第四,在温度较低时,生育酚的侧向扩散系数与磷脂的相当,但在350K时其扩散速度明显加快;在垂直方向生育酚的扩散速度很慢.
用分子動力學方法模擬瞭280,310和350 K-蔔a-生育酚在二豆蔻酞燐脂酞膽堿、二豆蔻酞燐脂酞乙醇胺、二硬脂酞燐脂酞膽堿和二硬脂酞燐脂酞乙醇胺雙層膜中的性質,包括瞭空間位置、氫鍵、取嚮和動力學性質,取得瞭如下的結論.第一,生育酚頭部的羥基一般位于脂雙層親疏水界麵的下方,升高溫度將促進羥基嚮膜雙層的中心移動,在350K時觀察到瞭在上下兩箇單層間的翻轉.第二,生育酚主要與燐脂的酯基形成氫鍵,幾乎不與燐脂酞乙醇胺的氨基形成氫鍵;比較生育酚與燐脂酞膽堿和乙醇胺形成的氫鍵後髮現,後者更穩定.第三,生育酚的頭部在膜中取嚮多變,與膜的法線夾角不固定,尾部的構象也很複雜.第四,在溫度較低時,生育酚的側嚮擴散繫數與燐脂的相噹,但在350K時其擴散速度明顯加快;在垂直方嚮生育酚的擴散速度很慢.
용분자동역학방법모의료280,310화350 K-복a-생육분재이두구태린지태담감、이두구태린지태을순알、이경지태린지태담감화이경지태린지태을순알쌍층막중적성질,포괄료공간위치、경건、취향화동역학성질,취득료여하적결론.제일,생육분두부적간기일반위우지쌍층친소수계면적하방,승고온도장촉진간기향막쌍층적중심이동,재350K시관찰도료재상하량개단층간적번전.제이,생육분주요여린지적지기형성경건,궤호불여린지태을순알적안기형성경건;비교생육분여린지태담감화을순알형성적경건후발현,후자경은정.제삼,생육분적두부재막중취향다변,여막적법선협각불고정,미부적구상야흔복잡.제사,재온도교저시,생육분적측향확산계수여린지적상당,단재350K시기확산속도명현가쾌;재수직방향생육분적확산속도흔만.
Molecular dynamics simulations of α-tocopherol in a number of saturated phospholipid bilayers were performed at 280, 310, and 350 K. The phospholipids contained either short acyl tails, i.e.,dimyristoylphosphatidylcholine and dimyristoylphosphatidylethanolamine or long acyl tails, namely distearoylphosphatidylcholineanddistearoylphosphatidylethanolamine.Thepreferentialposition,hydrogen bonding, orientation, and dynamic properties of α-tocopherol in the bilayers were examined in detail and several conclusions were made. First, the hydroxyl group of a-tocopherol generally remains beneath the interfacial region of the lipid bilayers and it shifts toward the bilayer mid-plane with an increase in temperature. At 350 K it flip-flops between the upper and lower leaflets in the four lipid bilayers. Second,α-tocopherol mainly forms hydrogen bonds with the carbonyl ester oxygen in the lipid head groups and hardly forms hydrogen bonds with the amino groups in the phosphatidylethanolamine (PE) bilayers.Hydrogen bonding with PEs is more stable than hydrogen bonding with phosphatidylcholines (PCs) at low temperatures. Third, a-tocopherol's head group has fluctuating tilt angles relative to the normal of the lipid bilayers and the tail has many different conformations. Fourth, the lateral diffusion rate of a-tocopherol is comparable to that of phospholipid molecules at low temperature and it diffuses much faster than lipids at 350 K. The diffusion rate in the direction perpendicular to the membrane surface is much slower than the lateral diffusion rate.