中西医结合学报
中西醫結閤學報
중서의결합학보
JOURNAL OF CHINESE INTEGRATIVE MDEICINE
2011年
9期
1005-1013
,共9页
关键词:白头翁属%人参%甘草%植物提取物%血管生成抑制剂%细胞周期蛋白A%黏着斑蛋白酪氨酸激酶类%体外研究
關鍵詞:白頭翁屬%人參%甘草%植物提取物%血管生成抑製劑%細胞週期蛋白A%黏著斑蛋白酪氨痠激酶類%體外研究
관건사:백두옹속%인삼%감초%식물제취물%혈관생성억제제%세포주기단백A%점착반단백락안산격매류%체외연구
Pulsatilla%Panax ginseng%Glycyrrhiza uralensis%plant extracts%angiogenesis inhibitors%cyclin A%focal adhesion protein-tyrosine kinases%in vitro
目的:本研究旨在证实朝鲜白头翁、人参和甘草的复合水提物(water extract of Pulsatilla koreana (Yabe ex Nakai) Nakai ex T.Mori.,Panax ginseng C.A.Meyer and Glycyrrhiza uralensis Fisch,WEPPG)的抗血管生成作用.方法:使用纤维母细胞生长因子致血管生成的人类脐静脉内皮细胞模型衡量细胞的增殖、黏附及迁移,同时进行细管形成实验及纤维母细胞生长因子致鸡胚绒毛尿囊膜血管生成实验检测WEPPG的抗血管生成作用.结果:WEPPG能够显著抑制纤维母细胞生长因子所致血管生成的人类脐静脉内皮细胞的增殖、黏附及迁移.信号蛋白分析显示多种蛋白表达变化,如细胞周期素A、p63、KIP2的上调及nibrin蛋白和黏着斑激酶的下调.与对照组相比,WEPPG显著减少了鸡胚绒毛尿囊膜血管生成.结论:本研究的结果证实了WEPPG的抗血管生成作用,这可能是这种药物具有抗癌功效的原因之一.
目的:本研究旨在證實朝鮮白頭翁、人參和甘草的複閤水提物(water extract of Pulsatilla koreana (Yabe ex Nakai) Nakai ex T.Mori.,Panax ginseng C.A.Meyer and Glycyrrhiza uralensis Fisch,WEPPG)的抗血管生成作用.方法:使用纖維母細胞生長因子緻血管生成的人類臍靜脈內皮細胞模型衡量細胞的增殖、黏附及遷移,同時進行細管形成實驗及纖維母細胞生長因子緻鷄胚絨毛尿囊膜血管生成實驗檢測WEPPG的抗血管生成作用.結果:WEPPG能夠顯著抑製纖維母細胞生長因子所緻血管生成的人類臍靜脈內皮細胞的增殖、黏附及遷移.信號蛋白分析顯示多種蛋白錶達變化,如細胞週期素A、p63、KIP2的上調及nibrin蛋白和黏著斑激酶的下調.與對照組相比,WEPPG顯著減少瞭鷄胚絨毛尿囊膜血管生成.結論:本研究的結果證實瞭WEPPG的抗血管生成作用,這可能是這種藥物具有抗癌功效的原因之一.
목적:본연구지재증실조선백두옹、인삼화감초적복합수제물(water extract of Pulsatilla koreana (Yabe ex Nakai) Nakai ex T.Mori.,Panax ginseng C.A.Meyer and Glycyrrhiza uralensis Fisch,WEPPG)적항혈관생성작용.방법:사용섬유모세포생장인자치혈관생성적인류제정맥내피세포모형형량세포적증식、점부급천이,동시진행세관형성실험급섬유모세포생장인자치계배융모뇨낭막혈관생성실험검측WEPPG적항혈관생성작용.결과:WEPPG능구현저억제섬유모세포생장인자소치혈관생성적인류제정맥내피세포적증식、점부급천이.신호단백분석현시다충단백표체변화,여세포주기소A、p63、KIP2적상조급nibrin단백화점착반격매적하조.여대조조상비,WEPPG현저감소료계배융모뇨낭막혈관생성.결론:본연구적결과증실료WEPPG적항혈관생성작용,저가능시저충약물구유항암공효적원인지일.
Objective:This study aimed to investigate the anti-angiogenic effects of the water extract of Pulsatilla koreana (Yabe ex Nakai) Nakai ex T.Mori.,Panax ginseng C.A.Meyer and Glycyrrhiza uralensis Fisch (WEPPG).Methods:The effects of WEPPG on fibroblast growth factor (bFGF)-induced angiogenesis were evaluated by human umbilical vein endothelial cell (HUVEC) proliferation,adhesion,and migration assays.Capillary tube formation of HUVECs and bFGF-induced chick chorioallantoic membrane (CAM) angiogenesis were also observed.WEPPG was used to treat the HUVECs and CAMs,and then various activities such as proliferation,adhesion,migration,capillary tube formation and cell cycle proteins were analyzed.Results:WEPPG significantly inhibited bFGF-induced HUVEC proliferation,adhesion,migration,and capillary tube formation.Signaling protein analysis showed up-regulated expressions of various proteins including cyclin A,p63 and KIP2 and down-regulated expressions of nibrin and focal adhesion kinase.The blood vessel formation in a CAM treated with WEPPG was markedly reduced compared with the control group.Conclusion:These results suggested that the inhibition of angiogenesis by WEPPG can be an action mechanism for its anti-cancer effects.
