CAJ | 학술논문

目的探讨抑癌候选基因Ndrg2在不同分化级别结直肠癌组织中的表达特点,分析其调控结直肠癌细胞分化的意义.方法收集50例不同分化级别结直肠癌组织标本,分别通过免疫组织化学染色及蛋白印迹分析检测Ndrg2的表达;以150点结直肠癌组织芯片大样本分析Ndrg2的表达特点.结合组织芯片相关病例信息进行统计学分析.结果 32例结直肠癌组织Ndrg2表达显著低于其自身癌旁组织及正常组织,癌旁组织中的表达也显著低于正常组织中的表达.组织芯片统计分析显示,不同分化程度结直肠癌组织Ndrg2的表达差异有统计学意义(P=0.005);而Ndrg2表达与患者的年龄、性别、肿瘤部位、结直肠癌浸润深度、淋巴转移及UICC分期无关.结论 Ndrg2可能参与调控结直肠癌细胞的分化过程.
목적 탐토억암후선기인Ndrg2재불동분화급별결직장암조직중적표체특점,분석기조공결직장암세포분화적의의.방법 수집50례불동분화급별결직장암조직표본,분별통과면역조직화학염색급단백인적분석검측Ndrg2적표체;이150점결직장암조직심편대양본분석Ndrg2적표체특점.결합조직심편상관병례신식진행통계학분석.결과 32례결직장암조직Ndrg2표체현저저우기자신암방조직급정상조직,암방조직중적표체야현저저우정상조직중적표체.조직심편통계분석현시,불동분화정도결직장암조직Ndrg2적표체차이유통계학의의(P=0.005);이Ndrg2표체여환자적년령、성별、종류부위、결직장암침윤심도、림파전이급UICC분기무관.결론 Ndrg2가능삼여조공결직장암세포적분화과정.
Objective To determine the expression of new candidate tumor suppressor gene N-Myc downstream-regulated gene 2(Ndrg2) in colorectal cancer with different differentiation, and analyze its clinical significance. Methods Specimens of 50 colorectal cancer patients with different differentiation were collected, Immunohistochemistry and Western blot were used to examine the expression of Ndrg2. Colorectal cancer tissue array in large scale was applied to analyze the expression of Ndrg2 and the statistics analysis was performed referring to the patients information of the array. Results Among 50 cases, Ndrg2 expression level of colorectal cancer was significantly lower in 32 cases as compared to adjacent and normal tissue of the same individual, while Ndrg2 expression of adjacent tissue was significantly lower than that of normal tissue. Ndrg2 protein levels increased from poor-differentiated to well-differentiated carcinomas (P=0.005). Conclusions The expression of Ndrg2 in different differentiated colorectal cancer tissues show a significant distinction. Ndrg2 may be involved in the regulation of differentiation in colorectal cancer.