CAJ | 학술논문

目的观察骨髓间充质干细胞(bone mesenchymal stem cell,BMSC)联合血管内皮生长因子(vascular endothelial growth factor,VEGF)基因治疗对家兔肢体缺血模型的疗效.方法切除新西兰兔右后肢全长股浅动脉并结扎股深动脉以建立兔后肢缺血模型,随机分为空质粒对照组(EP组)、骨髓间充质干细胞组(BMSC组)、VEGF基因治疗组(VEGF组)及联合治疗组(BV组),每组各8只.分别于治疗后28 d及30 d进行动脉造影及VEGF免疫组化染色.结果 EP组、BMSC组及VEGF组的新生血管计数组间比较差异无统计学意义(P＞0.05).BV组的新生血管计数较其余3组明显增加,差异有统计学意义(F=35.47,P＜O.01).BMSC组及VEGF组的VEGF免疫组化染色呈阳性表达,与EP组比较差异有统计学意义(F=764.32,P＜0.01).BV组的VEGF免疫组化染色呈强阳性表达,与其余3组比较差异有统计学意义(F =764.32,P＜0.01).结论 BMSC联合VEGF基因治疗兔肢体缺血可使VEGF获得稳定而有效的表达,从而改善肢体缺血.
목적 관찰골수간충질간세포(bone mesenchymal stem cell,BMSC)연합혈관내피생장인자(vascular endothelial growth factor,VEGF)기인치료대가토지체결혈모형적료효.방법 절제신서란토우후지전장고천동맥병결찰고심동맥이건립토후지결혈모형,수궤분위공질립대조조(EP조)、골수간충질간세포조(BMSC조)、VEGF기인치료조(VEGF조)급연합치료조(BV조),매조각8지.분별우치료후28 d급30 d진행동맥조영급VEGF면역조화염색.결과 EP조、BMSC조급VEGF조적신생혈관계수조간비교차이무통계학의의(P＞0.05).BV조적신생혈관계수교기여3조명현증가,차이유통계학의의(F=35.47,P＜O.01).BMSC조급VEGF조적VEGF면역조화염색정양성표체,여EP조비교차이유통계학의의(F=764.32,P＜0.01).BV조적VEGF면역조화염색정강양성표체,여기여3조비교차이유통계학의의(F =764.32,P＜0.01).결론 BMSC연합VEGF기인치료토지체결혈가사VEGF획득은정이유효적표체,종이개선지체결혈.
Objective To evaluate bone marrow mesenchymal stem cells combined with VEGF gene in the treatment of limb ischemia in rabbits.Methods The right hind limb ischemia model of New Zealand rabbit was established by superficial femoral artery excision and deep femoral artery ligation.Rabbits then were divided randomly into 4 groups: empty plasmid control group(EP group),bone marrow mesenchymal stem cells group(BMSC group),VEGF gene therapy group(VEGF group),combination bone mesenchymal stem cells and VEGF gene therapy group(BV group).There were 8 rabbits in each group.Angiogenesis was detected by arteriography on day 28 after treatment and expression of VEGF was detected by immunohistochemical staining on day 30 after treatment.Results There were no differences of collateral vessel count between the EP group,BMSC group and VEGF group.The collateral vessel count in BV group was higher than that of the other three groups.Immunohistochemistry of VEGF showed that the integrated optical density(IOD)in BMSC and VEGF groups increased significantly compared with the EP group; the IOD in BV group was the highest compared with the other three groups.Conclusions Combination bone marrow mesenchymal stem cells and VEGF gene in the treatment of limb ischemia in rabbits can obtain stable and effective expression of VEGF along with significant improvement of limb ischemia.