中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2010年
5期
367-370
,共4页
舒晓春%庞天骄%尹代婵%刘君静%朱丹华%叶礼红%鲁红云
舒曉春%龐天驕%尹代嬋%劉君靜%硃丹華%葉禮紅%魯紅雲
서효춘%방천교%윤대선%류군정%주단화%협례홍%로홍운
葡萄糖%骨质疏松%成骨细胞%骨髓基质干细胞
葡萄糖%骨質疏鬆%成骨細胞%骨髓基質榦細胞
포도당%골질소송%성골세포%골수기질간세포
Gluscose%Osteoporosis%Osteoblasts%Bone-marrow stromal cells
目的 体外观察不同葡萄糖浓度在促骨髓基质干细胞向成骨细胞分化过程中对细胞因子碱性磷酸酶、骨形成蛋白-2、转化生长因子β1表达的影响,并探讨其对骨代谢的作用机制.方法 无菌条件下从2周龄SD大鼠长骨骨髓中分离获取骨髓基质干细胞,采用全骨髓贴壁培养法对骨髓基质干细胞进行纯化、传代扩增,随后在不同糖浓度(5.5 mmol/L、25.0 mmol/L)干预下向成骨细胞诱导分化培养21 d,进行茜素红染色观察矿化结节并测定成骨细胞的标记物碱性磷酸酶、骨形成蛋白-2及转化生长因子β1表达,比较各组中成骨细胞分化的情况.组间比较采用单因素方差分析法.结果 25.0 mmol/L糖浓度组与5.5 mmol/L糖浓度组比较,钙结节形成比例分别为(45.3±0.7)%、(68.3±0.8)%,差异具有统计学意义(P＜0.05),碱性磷酸酶(0.350±0.020、0.563±0.043)、骨形成蛋白-2[(590±27)、(744±41)μg/L]及转化生长因子β1[(875±40)、(1188±52)μg/L]活性比较,差异具有统计学意义(均P＜0.05).结论 在高糖条件下,骨髓基质干细胞向成骨细胞的分化减弱,这可能是糖尿病性骨质疏松的重要机制之一.
目的 體外觀察不同葡萄糖濃度在促骨髓基質榦細胞嚮成骨細胞分化過程中對細胞因子堿性燐痠酶、骨形成蛋白-2、轉化生長因子β1錶達的影響,併探討其對骨代謝的作用機製.方法 無菌條件下從2週齡SD大鼠長骨骨髓中分離穫取骨髓基質榦細胞,採用全骨髓貼壁培養法對骨髓基質榦細胞進行純化、傳代擴增,隨後在不同糖濃度(5.5 mmol/L、25.0 mmol/L)榦預下嚮成骨細胞誘導分化培養21 d,進行茜素紅染色觀察礦化結節併測定成骨細胞的標記物堿性燐痠酶、骨形成蛋白-2及轉化生長因子β1錶達,比較各組中成骨細胞分化的情況.組間比較採用單因素方差分析法.結果 25.0 mmol/L糖濃度組與5.5 mmol/L糖濃度組比較,鈣結節形成比例分彆為(45.3±0.7)%、(68.3±0.8)%,差異具有統計學意義(P＜0.05),堿性燐痠酶(0.350±0.020、0.563±0.043)、骨形成蛋白-2[(590±27)、(744±41)μg/L]及轉化生長因子β1[(875±40)、(1188±52)μg/L]活性比較,差異具有統計學意義(均P＜0.05).結論 在高糖條件下,骨髓基質榦細胞嚮成骨細胞的分化減弱,這可能是糖尿病性骨質疏鬆的重要機製之一.
목적 체외관찰불동포도당농도재촉골수기질간세포향성골세포분화과정중대세포인자감성린산매、골형성단백-2、전화생장인자β1표체적영향,병탐토기대골대사적작용궤제.방법 무균조건하종2주령SD대서장골골수중분리획취골수기질간세포,채용전골수첩벽배양법대골수기질간세포진행순화、전대확증,수후재불동당농도(5.5 mmol/L、25.0 mmol/L)간예하향성골세포유도분화배양21 d,진행천소홍염색관찰광화결절병측정성골세포적표기물감성린산매、골형성단백-2급전화생장인자β1표체,비교각조중성골세포분화적정황.조간비교채용단인소방차분석법.결과 25.0 mmol/L당농도조여5.5 mmol/L당농도조비교,개결절형성비례분별위(45.3±0.7)%、(68.3±0.8)%,차이구유통계학의의(P＜0.05),감성린산매(0.350±0.020、0.563±0.043)、골형성단백-2[(590±27)、(744±41)μg/L]급전화생장인자β1[(875±40)、(1188±52)μg/L]활성비교,차이구유통계학의의(균P＜0.05).결론 재고당조건하,골수기질간세포향성골세포적분화감약,저가능시당뇨병성골질소송적중요궤제지일.
Objective To observe the effects of glucose on the expression of alkaline phosphatase ( ALP), bone morphogenetic protein-2 ( BMP-2 ) and transforming growth factor-beta 1 ( TGF-51 ) for rat bone marrow stromal cells (BMSCs) differentiation into osteoblastic(OB) and to investigate the mechanism of the impact on the bone metabolism from them. Methods Bone-marrow stromal cells were achieved from long bone marrow of rats under aseptic condition. After purification, passage and amplification by using the whole bone marrow adherence method, the BMSCs were interfered into osteoblasts with different doses of glucose concentration (5.5 mmol/L and 25.0 mmol/L) in the presence of the osteogenic medium. The rate of mineralization was examined by staining of mineralized nodules with Alizarin red S, and the difference of ALP, BMP-2 and TGF-[β1 between interblocks was examined by enzyme linked immunosorbent assay (ELISA) after 21 d of culture. One way analysis of variance was used for statistical analysis between groups.Results Compared with 5. 5 mmol/L glucose classic group, the rate of mineralization was cut down significantly in 25.0 mmol/L glucose classic group ( (45.3 ± 0.7)% vs ( 68.3 ± 0.8 ) %, P ＜ 0.05 ) , and the activity of ALP (0.350 ± 0.020, 0.563 ± 0.043 ), BMP-2 ( (590 ± 27 ), (744 ± 41 )μg/L) and TGF-β1 ((875 ±40),(1188 ±52)μg/L)were also cut down significantly (all P＜0.05). Conclusions High concentration glucose inhibits the differentiation of BMSCs into osteoblastics, which provids a potential mechanisms of diabetes-induced osteoporosis.
