中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
14期
957-960
,共4页
结肠炎,溃疡性%肠易激综合征%β防御素%肿瘤坏死因子α
結腸炎,潰瘍性%腸易激綜閤徵%β防禦素%腫瘤壞死因子α
결장염,궤양성%장역격종합정%β방어소%종류배사인자α
Colitis,ulcerative%Irritable bowel syndrome%beta-Defensins%Tumor necrosis factor-alpha
目的 研究β-防御素2(HBD-2)和肿瘤坏死因子α(TNF-α)在溃疡性结肠炎和肠易激综合征(腹泻型)患者结肠黏膜中的表达,及其在发病中的作用和相互关系.方法 收集30例溃疡性结肠炎患者、20例肠易激综合征(腹泻型)患者和10名健康对照的结肠黏膜组织,免疫组织化学法检测各组HBD-2和TNF-α蛋白表达.结果 免疫组织化学分析显示溃疡性结肠炎组HBD-2和TNF-α表达强度均高于肠易激综合征(腹泻型)组(z=-4.856,z=-3.987,均P＜0.01)和正常结肠黏膜组(z=-3.611,z=-3.248,均P＜0.01),肠易激综合征(腹泻型)组和正常结肠黏膜组HBD-2和TNF-α表达强度比较差异无统计学意义(z=-0.373,z=-0.032,均P＞0.05).轻、中、重度3组溃疡性结肠炎之间HBD-2和TNF-α的表达强度比较差异无统计学意义(x2=1.190、P＞0.05,X2=1.672、P＞0.05).结论 HBD-2和TNF-α的表达水平在溃疡性结肠炎结肠黏膜组织中明显升高,与肠易激综合征(腹泻型)关系不密切,与溃疡性结肠炎病情轻重关系不密切.
目的 研究β-防禦素2(HBD-2)和腫瘤壞死因子α(TNF-α)在潰瘍性結腸炎和腸易激綜閤徵(腹瀉型)患者結腸黏膜中的錶達,及其在髮病中的作用和相互關繫.方法 收集30例潰瘍性結腸炎患者、20例腸易激綜閤徵(腹瀉型)患者和10名健康對照的結腸黏膜組織,免疫組織化學法檢測各組HBD-2和TNF-α蛋白錶達.結果 免疫組織化學分析顯示潰瘍性結腸炎組HBD-2和TNF-α錶達彊度均高于腸易激綜閤徵(腹瀉型)組(z=-4.856,z=-3.987,均P＜0.01)和正常結腸黏膜組(z=-3.611,z=-3.248,均P＜0.01),腸易激綜閤徵(腹瀉型)組和正常結腸黏膜組HBD-2和TNF-α錶達彊度比較差異無統計學意義(z=-0.373,z=-0.032,均P＞0.05).輕、中、重度3組潰瘍性結腸炎之間HBD-2和TNF-α的錶達彊度比較差異無統計學意義(x2=1.190、P＞0.05,X2=1.672、P＞0.05).結論 HBD-2和TNF-α的錶達水平在潰瘍性結腸炎結腸黏膜組織中明顯升高,與腸易激綜閤徵(腹瀉型)關繫不密切,與潰瘍性結腸炎病情輕重關繫不密切.
목적 연구β-방어소2(HBD-2)화종류배사인자α(TNF-α)재궤양성결장염화장역격종합정(복사형)환자결장점막중적표체,급기재발병중적작용화상호관계.방법 수집30례궤양성결장염환자、20례장역격종합정(복사형)환자화10명건강대조적결장점막조직,면역조직화학법검측각조HBD-2화TNF-α단백표체.결과 면역조직화학분석현시궤양성결장염조HBD-2화TNF-α표체강도균고우장역격종합정(복사형)조(z=-4.856,z=-3.987,균P＜0.01)화정상결장점막조(z=-3.611,z=-3.248,균P＜0.01),장역격종합정(복사형)조화정상결장점막조HBD-2화TNF-α표체강도비교차이무통계학의의(z=-0.373,z=-0.032,균P＞0.05).경、중、중도3조궤양성결장염지간HBD-2화TNF-α적표체강도비교차이무통계학의의(x2=1.190、P＞0.05,X2=1.672、P＞0.05).결론 HBD-2화TNF-α적표체수평재궤양성결장염결장점막조직중명현승고,여장역격종합정(복사형)관계불밀절,여궤양성결장염병정경중관계불밀절.
Objective To detect the expression of human β-defensin 2(HBD-2)and tumor necrosis factor-α(TNF-α)in the colonic mucosae of ulcerative colitis(UC)patients and diarrheapredominant irritable bowel syndrome (IBS-D)patients and to evaluate the roles of HBD-2 and TNF-α in the pathogenesis and the relationship between them. Methods Immunohistochemistry was conducted on the biopsy samples of colonic mucosa from 30 UC patients, 20 IBS-D patients, and 10 normal persons to measure the expression of HBD-2 and TNF-α. Results The expression levels of HBD-2 and TNF-α in the colonic mncosa of UC were both significantly higher than those in the colonic mucosa of IBS-D (z=-4. 856, z=-3. 987, all P＜0. 01)and in the normal colonic mucosa(z=-3. 611, z=-3. 248, all P＜0. 01). But no significant differences were found between the colonic mucosa of IBS-D and the normal colonic mucosa in the intensity of the expressions of HBD-2 and TNF-α(z=-0. 373. z=-0. 032. all P＞0. 05). There were no significant differences in the intensity of the expressions of HBD-2 and TNF-α in colonic mucosa of among the mild, moderate, and severe UC(x2=1. 190、P＞0. 05, x2=1. 672、P＞0. 05). Conclusion The expressions of HBD-2 and TNF-α are significantly increased in UC, but are not correlated with IBS-D and the severity of clinical symptoms of UC patients.
