CAJ | 학술논문

目的制备抗人cTn I单克隆抗体，以便进一步制备AMI诊断制剂。方法用兔骨骼肌Tnc(sT-nC)制备ThC-Sepharose-4B亲和柱，直接从心肌组织中分离提纯人cTn I。结果所提纯的cTn I经SDS-PAGE分析已达电泳纯，其相对分子质量和氨基酸组成与文献报道相符。结论为建立AMI早期诊断试剂奠定了基础。
목적 제비항인cTn I단극륭항체，이편진일보제비AMI진단제제。방법 용토골격기Tnc(sT-nC)제비ThC-Sepharose-4B친화주，직접종심기조직중분리제순인cTn I。결과 소제순적cTn I경SDS-PAGE분석이체전영순，기상대분자질량화안기산조성여문헌보도상부。결론 위건립AMI조기진단시제전정료기출。
Objective To prepare the McAb to cardiac troponin I (cTn I ) for further preparation ofdiagnostic kit for acute myocardiac infarction(AMI). Methods TnC- Sepharose 4B affinity chromatographycolumn was prepared with rabbit skeletal muscles troponin C(sInC) and used for the direct purification of cTnI from human cardiac muscle tissue. Results SDS-PAGE proved that the eTn I was electrophoretic pure,and the molecular weight and amino acid component of it were consistent with that reported in documents. Con-clusion The study lays a foundation of developing the diagnostic kit for early AMI.